Detailed information of TA system TA149129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 4636246..4637234
• Replicon: chromosome
• Accession: NZ_CP048337
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: GZS12_RS22250
• Protein ID: WP_014640052.1
• Coordinates: 4636246..4636632 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: A0A0A1AFM1
• Locus tag: GZS12_RS22255
• Protein ID: WP_000458583.1
• Coordinates: 4636662..4637234 (-)
• Length: 191 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS22220	4631811..4632011	-	201	WP_021527487.1	helix-turn-helix domain-containing protein	-
GZS12_RS22225	4632102..4632776	+	675	WP_021530636.1	LexA family transcriptional regulator	-
GZS12_RS22230	4633444..4633806	+	363	WP_000135680.1	hypothetical protein	-
GZS12_RS22235	4633872..4634696	+	825	WP_023908886.1	DUF2303 family protein	-
GZS12_RS22240	4634915..4635667	+	753	WP_024946495.1	hypothetical protein	-
GZS12_RS22245	4635704..4635985	+	282	WP_001093916.1	pyocin activator PrtN family protein	-
GZS12_RS26110	4636033..4636206	-	174	WP_023908888.1	hypothetical protein	-
GZS12_RS22250	4636246..4636632	-	387	WP_014640052.1	hypothetical protein	Toxin
GZS12_RS22255	4636662..4637234	-	573	WP_000458583.1	SocA family protein	Antitoxin
GZS12_RS22260	4637470..4637592	-	123	WP_001300034.1	hypothetical protein	-
GZS12_RS22265	4637954..4638958	-	1005	WP_022646425.1	DUF2713 family protein	-
GZS12_RS22270	4639276..4639791	+	516	WP_000416263.1	zinc uptake transcriptional repressor Zur	-
GZS12_RS22275	4639833..4640042	-	210	WP_001296638.1	CsbD family protein	-
GZS12_RS22280	4640158..4641483	-	1326	WP_001361471.1	MATE family efflux transporter DinF	-
GZS12_RS22285	4641556..4642164	-	609	WP_000646078.1	transcriptional repressor LexA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espX4 / espX4	4594188..4651060	56872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin SocA-like, Panacea domain (27-137)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14465.40 Da
• Isoelectric Point: 5.4833

>T149129 WP_014640052.1 NZ_CP048337:c4636632-4636246 [Escherichia coli]
VLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHDPSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQ
RSGEISVHDDMPEATFNRILDGFDISDEVTPKNLKFKNKYCVSSIDDE

Nucleotide

• Length: 387 bp

>T149129 NZ_CP063932:298982-299899 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATAAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATATCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACACG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 191 a.a.
• Molecular weight: 21956.30 Da
• Isoelectric Point: 6.0283

>AT149129 WP_000458583.1 NZ_CP048337:c4637234-4636662 [Escherichia coli]
MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRKSILKHGRMIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYN
LIETNGHDVLLRSDPREMDADEVFDELSRADIRILDEIYSLYGHMNRFDLANMTHLESVCPEWHNPGNSRKPIDLKEMLI
SEGKSEDEANRIIGKMEESQKLKEFSLQLS

Nucleotide

• Length: 573 bp

>AT149129 NZ_CP063932:298218-298976 [Klebsiella variicola]
ATGCCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGCTGAGTATGGAAAATAAAGCGCT
GTTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
GATACCGCGTACTGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0A1AFM1