Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4511083..4511580 Replicon chromosome
Accession NZ_CP048337
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GZS12_RS21590 Protein ID WP_001173343.1
Coordinates 4511083..4511256 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GZS12_RS21595 Protein ID WP_000398619.1
Coordinates 4511284..4511580 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS21580 4507753..4509210 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
GZS12_RS21585 4509447..4510964 + 1518 WP_022646456.1 lysine--tRNA ligase -
GZS12_RS21590 4511083..4511256 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GZS12_RS21595 4511284..4511580 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GZS12_RS21600 4511809..4512081 - 273 WP_000405647.1 N-acetyltransferase -
GZS12_RS21605 4512093..4512323 - 231 WP_001683478.1 (4Fe-4S)-binding protein -
GZS12_RS21610 4512504..4514135 + 1632 WP_022646455.1 sensor histidine kinase -
GZS12_RS21615 4514132..4514851 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4476376..4511580 35204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T149128 WP_001173343.1 NZ_CP048337:c4511256-4511083 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T149128 NZ_CP063931:183041-183457 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGATGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCAGCCGGCGCCGTCCTGGTGACGAACAATGTGCGGGAGTTTGAGCGGGTGCCGGGCCTGATGCTGG
AAGACTGGGTGAATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT149128 WP_000398619.1 NZ_CP048337:c4511580-4511284 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT149128 NZ_CP063931:182814-183044 [Klebsiella pneumoniae]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACATGGGGCTCGTTCGCGCAACTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGCGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References