Detailed information of TA system TA149127

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4498200..4499035
• Replicon: chromosome
• Accession: NZ_CP048337
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PMM7
• Locus tag: GZS12_RS21535
• Protein ID: WP_000854720.1
• Coordinates: 4498658..4499035 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P8X0
• Locus tag: GZS12_RS21530
• Protein ID: WP_001539669.1
• Coordinates: 4498200..4498568 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS12_RS21495	4494426..4495123	+	698	Protein_4202	IS1 family transposase	-
GZS12_RS21500	4495120..4495569	+	450	WP_080700391.1	hypothetical protein	-
GZS12_RS21505	4495689..4495862	+	174	WP_000088744.1	DUF905 family protein	-
GZS12_RS21510	4495962..4496780	+	819	WP_023909039.1	DUF945 domain-containing protein	-
GZS12_RS21515	4496835..4497320	+	486	WP_000849596.1	antirestriction protein	-
GZS12_RS21520	4497336..4497812	+	477	WP_001186771.1	RadC family protein	-
GZS12_RS21525	4497899..4498120	+	222	WP_000692349.1	DUF987 domain-containing protein	-
GZS12_RS21530	4498200..4498568	+	369	WP_001539669.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS12_RS21535	4498658..4499035	+	378	WP_000854720.1	TA system toxin CbtA family protein	Toxin
GZS12_RS21540	4499032..4499520	+	489	WP_000777672.1	hypothetical protein	-
GZS12_RS21545	4499532..4499729	+	198	WP_000839238.1	DUF957 domain-containing protein	-
GZS12_RS21550	4499822..4500688	+	867	WP_001290180.1	DUF4942 domain-containing protein	-
GZS12_RS21555	4500760..4501023	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
GZS12_RS21560	4501020..4501346	+	327	WP_000779482.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GZS12_RS21565	4502229..4503767	+	1539	WP_023908864.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4476376..4511580	35204
	-	flank	IS/Tn	-	-	4494620..4495123	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14089.02 Da
• Isoelectric Point: 7.9087

>T149127 WP_000854720.1 NZ_CP048337:4498658-4499035 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T149127 NZ_CP063931:c176691-176386 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAAGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13614.39 Da
• Isoelectric Point: 5.9550

>AT149127 WP_001539669.1 NZ_CP048337:4498200-4498568 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSN
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT149127 NZ_CP063931:c176911-176693 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829FW05

Antitoxin

• AlphaFold DB: A0A829FYK7