Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3690419..3691037 Replicon chromosome
Accession NZ_CP048337
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GZS12_RS17575 Protein ID WP_001291435.1
Coordinates 3690819..3691037 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GZS12_RS17570 Protein ID WP_000344800.1
Coordinates 3690419..3690793 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS17560 3685508..3686701 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GZS12_RS17565 3686724..3689873 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
GZS12_RS17570 3690419..3690793 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GZS12_RS17575 3690819..3691037 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GZS12_RS17580 3691209..3691760 + 552 WP_000102574.1 maltose O-acetyltransferase -
GZS12_RS17585 3691876..3692346 + 471 WP_022645280.1 YlaC family protein -
GZS12_RS17590 3692510..3694060 + 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GZS12_RS17595 3694102..3694455 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GZS12_RS17605 3694834..3695145 + 312 WP_000409911.1 MGMT family protein -
GZS12_RS17610 3695176..3695748 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149122 WP_001291435.1 NZ_CP048337:3690819-3691037 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149122 NZ_CP063931:c6655-6128 [Klebsiella pneumoniae]
GTGATGCAAGAGCTGGCGATAGAGATGATCAACGACGAATTTGACTATGACATAGGCGAATTCGATTGTGGAGAAGAAAC
CTTAAATACATTTTTGAAAGACCATCTCAAGCGGCAGCATGATGGGCAAATTTTAAAAGGTTATGTATTAGTAACCAAAG
AACAGAAGCCTAAGCTGTTAGGCTATTACACATTATCCGGGAGCTGTTTCGAGAGAAAAATGCTCCCGTCAAAAACACAG
CAAAGAAAAATCCCTTATCAGAATGCGCCAAGCATTACTTTGGGACGCTTGGCTATTGACAAATCAATTCAAGGTCAGGG
ATGGGGTGAATTGCTAGTAACTCACGCAATGAATGTCGTCTGGGATGCATCTAAGGCCGTAGGAATATATGGTCTCTTCG
TGGAAGCTCTGAACGATAAAGCTAAGCGCTTCTATACCAGATTAGATTTCATACAGCTCGTTGGTGAGAATAGTAATCTG
CTATTTTACCCGACAAAATCTATTGAAAAAATCTTTACCGAAGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149122 WP_000344800.1 NZ_CP048337:3690419-3690793 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149122 NZ_CP063931:c6932-6663 [Klebsiella pneumoniae]
ATGTCCGCACTGAGAAAGCAGCGCATAGATCTAAGATTAACTGATGACGACAAAAGCTTAATCGAAGAAGCCGCGGCAAT
GTCAAACCAGACTATTACACAGTTTGTAGTTGCCAGTGCCTCAGAACGTGCAGCGGAAGTGATTGAACAACATCGCAGAA
TGGTTCTAAACGAGGAGTCTTGGAACCTTGTCATGGATGCGATCGCAAACCCTGCTGCGCCGAATGAGCGGCTGAAGCGA
GCAGCGAAGCGTCTACAAAAAATGAGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References