Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2591933..2592571 Replicon chromosome
Accession NZ_CP048337
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GZS12_RS12250 Protein ID WP_000813794.1
Coordinates 2592395..2592571 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GZS12_RS12245 Protein ID WP_001270286.1
Coordinates 2591933..2592349 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS12225 2587085..2588026 - 942 WP_022645667.1 ABC transporter permease -
GZS12_RS12230 2588027..2589040 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
GZS12_RS12235 2589058..2590203 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
GZS12_RS12240 2590448..2591854 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
GZS12_RS12245 2591933..2592349 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GZS12_RS12250 2592395..2592571 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GZS12_RS12255 2592793..2593023 + 231 WP_000491567.1 DUF2554 family protein -
GZS12_RS12260 2593115..2595076 - 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GZS12_RS12265 2595149..2595685 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
GZS12_RS12270 2595738..2596949 + 1212 WP_071788120.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T149118 WP_000813794.1 NZ_CP048337:c2592571-2592395 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T149118 NZ_CP063930:3405131-3405541 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT149118 WP_001270286.1 NZ_CP048337:c2592349-2591933 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT149118 NZ_CP063930:3404884-3405150 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References