Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1145357..1145940 Replicon chromosome
Accession NZ_CP048337
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag GZS12_RS05435 Protein ID WP_000254750.1
Coordinates 1145605..1145940 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GZS12_RS05430 Protein ID WP_000581937.1
Coordinates 1145357..1145605 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS05420 1141696..1142997 + 1302 WP_000046818.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
GZS12_RS05425 1143045..1145279 + 2235 WP_000226815.1 GTP pyrophosphokinase -
GZS12_RS05430 1145357..1145605 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GZS12_RS05435 1145605..1145940 + 336 WP_000254750.1 endoribonuclease MazF Toxin
GZS12_RS05440 1146012..1146803 + 792 WP_001071641.1 nucleoside triphosphate pyrophosphohydrolase -
GZS12_RS05445 1147031..1148668 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GZS12_RS05450 1148755..1150053 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T149107 WP_000254750.1 NZ_CP048337:1145605-1145940 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T149107 NZ_CP063929:c66752-66594 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT149107 WP_000581937.1 NZ_CP048337:1145357-1145605 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT149107 NZ_CP063929:c66827-66796 [Klebsiella pneumoniae]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References