Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 994467..995121 Replicon chromosome
Accession NZ_CP048337
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GZS12_RS04755 Protein ID WP_000244781.1
Coordinates 994714..995121 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GZS12_RS04750 Protein ID WP_000354046.1
Coordinates 994467..994733 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS12_RS04730 990545..991978 - 1434 WP_022646090.1 6-phospho-beta-glucosidase BglA -
GZS12_RS04735 992023..992334 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
GZS12_RS04740 992498..993157 + 660 WP_000250269.1 hemolysin III family protein -
GZS12_RS04745 993234..994214 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
GZS12_RS04750 994467..994733 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GZS12_RS04755 994714..995121 + 408 WP_000244781.1 protein YgfX Toxin
GZS12_RS04760 995161..995682 - 522 WP_001055874.1 flavodoxin FldB -
GZS12_RS04765 995794..996690 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GZS12_RS04770 996715..997425 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GZS12_RS04775 997431..999164 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T149106 WP_000244781.1 NZ_CP048337:994714-995121 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T149106 NZ_CP063929:25561-25767 [Klebsiella pneumoniae]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT149106 WP_000354046.1 NZ_CP048337:994467-994733 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT149106 NZ_CP063929:c25574-25513 [Klebsiella pneumoniae]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References