Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 117600..118243 Replicon plasmid p010_A
Accession NZ_CP048331
Organism Escherichia coli strain 10

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag GZS13_RS24445 Protein ID WP_001034044.1
Coordinates 117827..118243 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag GZS13_RS24440 Protein ID WP_001261286.1
Coordinates 117600..117830 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS13_RS24425 112737..112967 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GZS13_RS24430 112964..113380 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
GZS13_RS24435 113425..117219 - 3795 WP_001144732.1 hypothetical protein -
GZS13_RS24440 117600..117830 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GZS13_RS24445 117827..118243 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
GZS13_RS24450 118318..119883 + 1566 WP_162656972.1 AAA family ATPase -
GZS13_RS24455 119868..120890 + 1023 WP_000361402.1 helicase UvrD -
GZS13_RS24460 121144..121841 - 698 Protein_142 IS1-like element IS1A family transposase -
GZS13_RS24465 122197..123111 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / sitABCD - 1..129675 129675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T149101 WP_001034044.1 NZ_CP048331:117827-118243 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T149101 NZ_CP063927:5164494-5164596 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT149101 WP_001261286.1 NZ_CP048331:117600-117830 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT149101 NZ_CP063927:c5164603-5164458 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References