Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 112737..113380 Replicon plasmid p010_A
Accession NZ_CP048331
Organism Escherichia coli strain 10

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag GZS13_RS24430 Protein ID WP_001034046.1
Coordinates 112964..113380 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag GZS13_RS24425 Protein ID WP_001261278.1
Coordinates 112737..112967 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS13_RS24395 108269..108574 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
GZS13_RS24400 108576..108794 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
GZS13_RS24405 109340..109852 + 513 WP_000151784.1 hypothetical protein -
GZS13_RS24410 109886..111019 - 1134 WP_000545986.1 DUF3800 domain-containing protein -
GZS13_RS24415 111186..111959 - 774 WP_000905949.1 hypothetical protein -
GZS13_RS24420 111972..112472 - 501 WP_000528932.1 hypothetical protein -
GZS13_RS24425 112737..112967 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GZS13_RS24430 112964..113380 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
GZS13_RS24435 113425..117219 - 3795 WP_001144732.1 hypothetical protein -
GZS13_RS24440 117600..117830 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GZS13_RS24445 117827..118243 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / sitABCD - 1..129675 129675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T149100 WP_001034046.1 NZ_CP048331:112964-113380 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T149100 NZ_CP063927:4062652-4063062 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGATGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT149100 WP_001261278.1 NZ_CP048331:112737-112967 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT149100 NZ_CP063927:4062405-4062671 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References