Detailed information of TA system TA149099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 108269..108794
• Replicon: plasmid p010_A
• Accession: NZ_CP048331
• Organism: Escherichia coli strain 10

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: GZS13_RS24395
• Protein ID: WP_001159868.1
• Coordinates: 108269..108574 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: GZS13_RS24400
• Protein ID: WP_000813634.1
• Coordinates: 108576..108794 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS13_RS24380	104179..105345	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
GZS13_RS24385	105933..106688	-	756	WP_000852146.1	replication initiation protein RepE	-
GZS13_RS24390	107462..108268	-	807	WP_000016982.1	site-specific integrase	-
GZS13_RS24395	108269..108574	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
GZS13_RS24400	108576..108794	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GZS13_RS24405	109340..109852	+	513	WP_000151784.1	hypothetical protein	-
GZS13_RS24410	109886..111019	-	1134	WP_000545986.1	DUF3800 domain-containing protein	-
GZS13_RS24415	111186..111959	-	774	WP_000905949.1	hypothetical protein	-
GZS13_RS24420	111972..112472	-	501	WP_000528932.1	hypothetical protein	-
GZS13_RS24425	112737..112967	+	231	WP_001261278.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GZS13_RS24430	112964..113380	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / blaTEM-1B / sitABCD	-	1..129675	129675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T149099 WP_001159868.1 NZ_CP048331:c108574-108269 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T149099 NZ_CP063927:3624800-3625147 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT149099 WP_000813634.1 NZ_CP048331:c108794-108576 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT149099 NZ_CP063927:3625147-3625446 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2