Detailed information of TA system TA149075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 992653..993236
• Replicon: chromosome
• Accession: NZ_CP048330
• Organism: Escherichia coli strain 10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: GZS13_RS04805
• Protein ID: WP_000254738.1
• Coordinates: 992901..993236 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: GZS13_RS04800
• Protein ID: WP_000581937.1
• Coordinates: 992653..992901 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS13_RS04790	988992..990293	+	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
GZS13_RS04795	990341..992575	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
GZS13_RS04800	992653..992901	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GZS13_RS04805	992901..993236	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
GZS13_RS04810	993307..994098	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
GZS13_RS04815	994326..995963	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
GZS13_RS04820	996051..997349	+	1299	WP_162656826.1	phosphopyruvate hydratase	-
GZS13_RS04825	997409..998167	-	759	Protein_944	YgcG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T149075 WP_000254738.1 NZ_CP048330:992901-993236 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T149075 NZ_CP063925:c1907977-1907609 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT149075 WP_000581937.1 NZ_CP048330:992653-992901 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT149075 NZ_CP063925:c1908195-1907974 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4