Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 886627..887281 Replicon chromosome
Accession NZ_CP048330
Organism Escherichia coli strain 10

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GZS13_RS04290 Protein ID WP_000244777.1
Coordinates 886874..887281 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GZS13_RS04285 Protein ID WP_000354046.1
Coordinates 886627..886893 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS13_RS04260 881796..882539 + 744 WP_000951964.1 SDR family oxidoreductase -
GZS13_RS04265 882596..884029 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
GZS13_RS04270 884074..884385 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GZS13_RS04275 884549..885208 + 660 WP_000250274.1 hemolysin III family protein -
GZS13_RS04280 885404..886384 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GZS13_RS04285 886627..886893 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GZS13_RS04290 886874..887281 + 408 WP_000244777.1 toxin CptA Toxin
GZS13_RS04295 887321..887842 - 522 WP_001055874.1 flavodoxin FldB -
GZS13_RS04300 887954..888850 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GZS13_RS04305 888875..889585 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GZS13_RS04310 889591..891324 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T149074 WP_000244777.1 NZ_CP048330:886874-887281 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T149074 NZ_CP063925:c1464121-1463636 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT149074 WP_000354046.1 NZ_CP048330:886627-886893 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT149074 NZ_CP063925:c1464411-1464118 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAACCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGCGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References