Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3646968..3647551 Replicon chromosome
Accession NZ_CP048326
Organism Escherichia coli strain 61

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag GZS14_RS17620 Protein ID WP_000254745.1
Coordinates 3646968..3647303 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3P5DUY8
Locus tag GZS14_RS17625 Protein ID WP_074502498.1
Coordinates 3647303..3647551 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS14_RS17600 3642437..3642799 + 363 WP_000034929.1 hypothetical protein -
GZS14_RS17605 3642855..3644153 - 1299 WP_000036723.1 phosphopyruvate hydratase -
GZS14_RS17610 3644241..3645878 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GZS14_RS17615 3646106..3646897 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GZS14_RS17620 3646968..3647303 - 336 WP_000254745.1 endoribonuclease MazF Toxin
GZS14_RS17625 3647303..3647551 - 249 WP_074502498.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GZS14_RS17630 3647629..3649863 - 2235 WP_000226815.1 GTP pyrophosphokinase -
GZS14_RS17635 3649911..3651212 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T149066 WP_000254745.1 NZ_CP048326:c3647303-3646968 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T149066 NZ_CP063922:c4308534-4308316 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9359.72 Da        Isoelectric Point: 4.4791

>AT149066 WP_074502498.1 NZ_CP048326:c3647551-3647303 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGESKDKE
VW

Download         Length: 249 bp

>AT149066 NZ_CP063922:c4308935-4308561 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A3P5DUY8

References