Detailed information of TA system TA149063

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2860465..2861300
• Replicon: chromosome
• Accession: NZ_CP048326
• Organism: Escherichia coli strain 61

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GZS14_RS13930
• Protein ID: WP_042046912.1
• Coordinates: 2860923..2861300 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GZS14_RS13925
• Protein ID: WP_073961352.1
• Coordinates: 2860465..2860833 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS14_RS13895	2855863..2857404	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
GZS14_RS13900	2857575..2857739	+	165	WP_162676511.1	DUF905 family protein	-
GZS14_RS13905	2857894..2858712	+	819	WP_162676475.1	DUF945 domain-containing protein	-
GZS14_RS13910	2859054..2859527	+	474	WP_000855059.1	antirestriction protein	-
GZS14_RS13915	2859542..2860018	+	477	WP_001186711.1	RadC family protein	-
GZS14_RS13920	2860081..2860302	+	222	WP_000692345.1	DUF987 domain-containing protein	-
GZS14_RS13925	2860465..2860833	+	369	WP_073961352.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS14_RS13930	2860923..2861300	+	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
GZS14_RS13935	2861297..2861500	+	204	WP_000761705.1	hypothetical protein	-
GZS14_RS13940	2861513..2861626	+	114	WP_032140621.1	DUF957 domain-containing protein	-
GZS14_RS13945	2862174..2863387	+	1214	WP_139371347.1	IS3 family transposase	-
GZS14_RS13950	2863737..2864126	+	390	WP_077875581.1	transposase	-
GZS14_RS13960	2864580..2864762	+	183	WP_024198358.1	ethanolamine utilization protein	-
GZS14_RS13965	2864863..2865192	-	330	WP_000450409.1	DUF496 family protein	-
GZS14_RS13970	2865364..2865882	-	519	Protein_2732	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ugd / manB / manC / gnd / wcaJ / wcaG / gmd / wza / wzi / cpsACP / galF	2832404..2922543	90139
	-	inside	IScluster/Tn	-	-	2855102..2869649	14547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T149063 WP_042046912.1 NZ_CP048326:2860923-2861300 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T149063 NZ_CP063922:3115925-3116308 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13444.17 Da
• Isoelectric Point: 6.2050

>AT149063 WP_073961352.1 NZ_CP048326:2860465-2860833 [Escherichia coli]
VSDTLSGATHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT149063 NZ_CP063922:3115683-3115925 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA