Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1111957..1112794 Replicon chromosome
Accession NZ_CP048326
Organism Escherichia coli strain 61

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag GZS14_RS05345 Protein ID WP_000227784.1
Coordinates 1111957..1112499 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GZS14_RS05350 Protein ID WP_001297137.1
Coordinates 1112483..1112794 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS14_RS05325 1107496..1108407 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
GZS14_RS05330 1108575..1109066 + 492 WP_001138904.1 nucleotide binding protein YajQ -
GZS14_RS05335 1109194..1110558 - 1365 WP_001000978.1 MFS transporter -
GZS14_RS05340 1110966..1111901 + 936 WP_001297127.1 sel1 repeat family protein -
GZS14_RS05345 1111957..1112499 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
GZS14_RS05350 1112483..1112794 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GZS14_RS05355 1112979..1113869 - 891 WP_000971336.1 heme o synthase -
GZS14_RS05360 1113881..1114210 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GZS14_RS05365 1114210..1114824 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GZS14_RS05370 1114814..1116805 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GZS14_RS05375 1116827..1117774 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T149052 WP_000227784.1 NZ_CP048326:c1112499-1111957 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T149052 NZ_CP063921:2540487-2540834 [Klebsiella variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT149052 WP_001297137.1 NZ_CP048326:c1112794-1112483 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT149052 NZ_CP063921:2540834-2541133 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCACTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References