Detailed information of TA system TA149046

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4100942..4101774
• Replicon: chromosome
• Accession: NZ_CP048322
• Organism: Escherichia coli strain 64

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: GZS15_RS19720
• Protein ID: WP_000854765.1
• Coordinates: 4100942..4101316 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: GZS15_RS19725
• Protein ID: WP_001295723.1
• Coordinates: 4101406..4101774 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS15_RS19680	4096119..4096289	-	171	Protein_3842	fimbrial protein	-
GZS15_RS19685	4096344..4097041	+	698	Protein_3843	IS1 family transposase	-
GZS15_RS19695	4097417..4097575	-	159	WP_001467148.1	hypothetical protein	-
GZS15_RS19700	4097675..4097851	-	177	WP_000839290.1	DUF957 domain-containing protein	-
GZS15_RS19705	4097975..4098721	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
GZS15_RS19710	4098736..4100277	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
GZS15_RS19715	4100418..4100945	-	528	WP_123002132.1	hypothetical protein	-
GZS15_RS19720	4100942..4101316	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
GZS15_RS19725	4101406..4101774	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS15_RS19730	4101937..4102158	-	222	WP_000692311.1	DUF987 domain-containing protein	-
GZS15_RS19735	4102221..4102697	-	477	WP_001186774.1	RadC family protein	-
GZS15_RS19740	4102713..4103198	-	486	WP_000849588.1	antirestriction protein	-
GZS15_RS19745	4103253..4104071	-	819	WP_001234620.1	DUF945 domain-containing protein	-
GZS15_RS19750	4104171..4104404	-	234	WP_001119729.1	DUF905 family protein	-
GZS15_RS19755	4104483..4104938	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T149046 WP_000854765.1 NZ_CP048322:c4101316-4100942 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T149046 NZ_CP063920:c9902-9579 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAGCTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACAATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GGCGCCCGGGGATGGGTAAGCGAGGTGGGGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGATCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT149046 WP_001295723.1 NZ_CP048322:c4101774-4101406 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT149046 NZ_CP063920:c9530-9231 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCTGC
AGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTGCAG
AAGTGGTGGGTGCCAGCGTTGACCTTGTCCGCAGCTGGGAACAGCAACGGCGTATTCCGTCGGGTATAGCGCTGAAAATG
CTCTGCCTCATCGAGCGTGAGCCTGCACTGATTGATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52