Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3647853..3648436 Replicon chromosome
Accession NZ_CP048322
Organism Escherichia coli strain 64

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag GZS15_RS17625 Protein ID WP_000254745.1
Coordinates 3647853..3648188 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3P5DUY8
Locus tag GZS15_RS17630 Protein ID WP_074502498.1
Coordinates 3648188..3648436 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS15_RS17605 3643322..3643684 + 363 WP_000034929.1 hypothetical protein -
GZS15_RS17610 3643740..3645038 - 1299 WP_000036723.1 phosphopyruvate hydratase -
GZS15_RS17615 3645126..3646763 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GZS15_RS17620 3646991..3647782 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GZS15_RS17625 3647853..3648188 - 336 WP_000254745.1 endoribonuclease MazF Toxin
GZS15_RS17630 3648188..3648436 - 249 WP_074502498.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GZS15_RS17635 3648514..3650748 - 2235 WP_000226815.1 GTP pyrophosphokinase -
GZS15_RS17640 3650796..3652097 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T149044 WP_000254745.1 NZ_CP048322:c3648188-3647853 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T149044 NZ_CP063919:c4814831-4814658 [Klebsiella pneumoniae]
GTGAAACAGCGCGAATTCCAGCGCTGGTTGGAATCTCAGGGGGTCGAAATCACTGACGGTGCAAAACATCTGAAGTTGAG
ACTGAACGGGAAGCGTAGCGTCATGCCTCGCCACCCGGGATCCGAGATTGTAGAACAGCTTCGTAAAGCTATTCTCAAAC
AACTAGGCCTGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9359.72 Da        Isoelectric Point: 4.4791

>AT149044 WP_074502498.1 NZ_CP048322:c3648436-3648188 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGESKDKE
VW

Download         Length: 249 bp

>AT149044 NZ_CP063919:c4814599-4814189 [Klebsiella pneumoniae]
ATGCGATATCCTGTAAACCTAAGTAAAGCTGAAGAAGGCGGATTCTTCGTAGAATTCCCGGATATACCTGAAGCTCTTAC
GCAGGGTGATACCAAAGAAGAAGCGCTTGATAACGCACTTGACGCTTTGGTAACGGCTTTCGAGTTTTACTTCGAAGATG
GAGATAGGATTCCGGAGCCTGGTGTACCGACAGGAGACTATGTAGAGTTACCGTTAAGTATTGAAGCTAAAGTATTAATG
TTAAATGCCTTTATTGAATCAAAATTGAGCCAAACTGAACTGGCTGCAAAAATGAATGTTAAAAAACAGGAAGTTACAAG
GTTGTTTGACCTTAAACATTCAACAAAAATTGATACAATTCAAAAGGCTGTCAACTCACTTGGACATAGAATGGAGTTAA
AGTATGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A3P5DUY8

References