Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3362247..3362872 Replicon chromosome
Accession NZ_CP048322
Organism Escherichia coli strain 64

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag GZS15_RS16225 Protein ID WP_000911329.1
Coordinates 3362247..3362645 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GZS15_RS16230 Protein ID WP_000450524.1
Coordinates 3362645..3362872 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS15_RS16205 3357335..3358315 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
GZS15_RS16210 3358435..3359133 - 699 WP_000679812.1 esterase -
GZS15_RS16215 3359207..3361222 - 2016 WP_074683898.1 tRNA cytosine(34) acetyltransferase TmcA -
GZS15_RS16220 3361237..3362100 - 864 WP_001267507.1 neutral zinc metallopeptidase -
GZS15_RS16225 3362247..3362645 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
GZS15_RS16230 3362645..3362872 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GZS15_RS16235 3363028..3363741 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GZS15_RS16240 3363954..3364988 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
GZS15_RS16245 3365005..3365883 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GZS15_RS16250 3366029..3366601 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GZS15_RS16255 3366601..3367071 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3357335..3358315 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T149043 WP_000911329.1 NZ_CP048322:c3362645-3362247 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T149043 NZ_CP063919:c4719229-4719127 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT149043 WP_000450524.1 NZ_CP048322:c3362872-3362645 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT149043 NZ_CP063919:4719120-4719265 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References