Detailed information of TA system TA149041

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2861244..2862079
• Replicon: chromosome
• Accession: NZ_CP048322
• Organism: Escherichia coli strain 64

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GZS15_RS13935
• Protein ID: WP_042046912.1
• Coordinates: 2861702..2862079 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GZS15_RS13930
• Protein ID: WP_073961352.1
• Coordinates: 2861244..2861612 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS15_RS13900	2856641..2858182	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
GZS15_RS13905	2858353..2858517	+	165	WP_162676511.1	DUF905 family protein	-
GZS15_RS13910	2858672..2859490	+	819	WP_162676475.1	DUF945 domain-containing protein	-
GZS15_RS13915	2859832..2860305	+	474	WP_000855059.1	antirestriction protein	-
GZS15_RS13920	2860321..2860797	+	477	WP_001186774.1	RadC family protein	-
GZS15_RS13925	2860860..2861081	+	222	WP_000692345.1	DUF987 domain-containing protein	-
GZS15_RS13930	2861244..2861612	+	369	WP_073961352.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS15_RS13935	2861702..2862079	+	378	WP_042046912.1	TA system toxin CbtA family protein	Toxin
GZS15_RS13940	2862076..2862279	+	204	WP_000761705.1	hypothetical protein	-
GZS15_RS13945	2862292..2862405	+	114	WP_032140621.1	DUF957 domain-containing protein	-
GZS15_RS13950	2862953..2864166	+	1214	WP_139371347.1	IS3 family transposase	-
GZS15_RS13955	2864516..2864905	+	390	WP_077875581.1	transposase	-
GZS15_RS13965	2865359..2865541	+	183	WP_024198358.1	ethanolamine utilization protein	-
GZS15_RS13970	2865642..2865971	-	330	WP_000450409.1	DUF496 family protein	-
GZS15_RS13975	2866143..2866661	-	519	Protein_2733	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2852856..2864166	11310
	-	inside	IScluster/Tn	-	-	2855880..2870428	14548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14020.96 Da
• Isoelectric Point: 7.8276

>T149041 WP_042046912.1 NZ_CP048322:2861702-2862079 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T149041 NZ_CP063919:c2589407-2589189 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATTCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13444.17 Da
• Isoelectric Point: 6.2050

>AT149041 WP_073961352.1 NZ_CP048322:2861244-2861612 [Escherichia coli]
VSDTLSGATHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT149041 NZ_CP063919:c2589808-2589434 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG