Detailed information of TA system TA149032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2276100..2277688
• Replicon: chromosome
• Accession: NZ_CP048322
• Organism: Escherichia coli strain 64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9XU38
• Locus tag: GZS15_RS11015
• Protein ID: WP_001125455.1
• Coordinates: 2276100..2277422 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GZS15_RS11020
• Protein ID: WP_001296726.1
• Coordinates: 2277422..2277688 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS15_RS10995	2271347..2271544	-	198	WP_000543389.1	two-component system connector SafA	-
GZS15_RS11000	2271792..2274071	-	2280	WP_001460947.1	acid resistance putative oxidoreductase YdeP	-
GZS15_RS11005	2274357..2275054	-	698	Protein_2157	IS1 family transposase	-
GZS15_RS11010	2275580..2275788	-	209	Protein_2158	helix-turn-helix transcriptional regulator	-
GZS15_RS11015	2276100..2277422	-	1323	WP_001125455.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GZS15_RS11020	2277422..2277688	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GZS15_RS11025	2277897..2279297	-	1401	WP_139300234.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2274357..2274734	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49321.93 Da
• Isoelectric Point: 8.8753

>T149032 WP_001125455.1 NZ_CP048322:c2277422-2276100 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T149032 NZ_CP063918:202769-203092 [Klebsiella variicola]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAGCTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACAATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GGCGCCCGGGGATGGGTAAGCGAGGTGGGGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGATCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT149032 WP_001296726.1 NZ_CP048322:c2277688-2277422 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT149032 NZ_CP063918:203141-203443 [Klebsiella variicola]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGTGCCAGCGTTGACCTTGTCCGCAGCTGGGAACAGCAACGGCGTATTCCGTCGGGTATAGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTGATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: U9XU38