Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1146253..1146871 Replicon chromosome
Accession NZ_CP048322
Organism Escherichia coli strain 64

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GZS15_RS05515 Protein ID WP_001291435.1
Coordinates 1146253..1146471 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GZS15_RS05520 Protein ID WP_000344800.1
Coordinates 1146497..1146871 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS15_RS05480 1141543..1142115 + 573 WP_000779826.1 lipoprotein -
GZS15_RS05485 1142146..1142457 - 312 WP_000409911.1 MGMT family protein -
GZS15_RS05495 1142836..1143189 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GZS15_RS05500 1143231..1144781 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GZS15_RS05505 1144945..1145415 - 471 WP_000136192.1 YlaC family protein -
GZS15_RS05510 1145531..1146082 - 552 WP_000102564.1 maltose O-acetyltransferase -
GZS15_RS05515 1146253..1146471 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GZS15_RS05520 1146497..1146871 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GZS15_RS05525 1147417..1150566 - 3150 WP_162676438.1 efflux RND transporter permease AcrB -
GZS15_RS05530 1150589..1151782 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149031 WP_001291435.1 NZ_CP048322:c1146471-1146253 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T149031 NZ_CP063918:c5424-5008 [Klebsiella variicola]
ATGAAGAAAACCTGGATGCTCGATACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATCGTGGTCTCGGCCGTGACGTACGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT149031 WP_000344800.1 NZ_CP048322:c1146871-1146497 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT149031 NZ_CP063918:c5651-5421 [Klebsiella variicola]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCAGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References