Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 884630..885284 Replicon chromosome
Accession NZ_CP048322
Organism Escherichia coli strain 64

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GZS15_RS04275 Protein ID WP_000244772.1
Coordinates 884877..885284 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GZS15_RS04270 Protein ID WP_000354046.1
Coordinates 884630..884896 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS15_RS04245 879918..880661 + 744 WP_000951964.1 SDR family oxidoreductase -
GZS15_RS04250 880718..882151 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
GZS15_RS04255 882196..882507 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GZS15_RS04260 882671..883330 + 660 WP_000250274.1 hemolysin III family protein -
GZS15_RS04265 883407..884387 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GZS15_RS04270 884630..884896 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GZS15_RS04275 884877..885284 + 408 WP_000244772.1 protein YgfX Toxin
GZS15_RS04280 885324..885845 - 522 WP_001055874.1 flavodoxin FldB -
GZS15_RS04285 885957..886853 + 897 WP_052907629.1 site-specific tyrosine recombinase XerD -
GZS15_RS04290 886878..887588 + 711 WP_000715218.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GZS15_RS04295 887594..889327 + 1734 WP_052907630.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T149029 WP_000244772.1 NZ_CP048322:884877-885284 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T149029 NZ_CP063917:c4602752-4602650 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT149029 WP_000354046.1 NZ_CP048322:884630-884896 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT149029 NZ_CP063917:4602644-4602788 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References