Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4277321..4277916 Replicon chromosome
Accession NZ_CP048310
Organism Escherichia coli strain 32-4

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag GZS16_RS20475 Protein ID WP_000239579.1
Coordinates 4277321..4277671 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag GZS16_RS20480 Protein ID WP_001223208.1
Coordinates 4277665..4277916 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS16_RS20455 4272767..4273789 - 1023 WP_001295197.1 ABC transporter permease -
GZS16_RS20460 4273803..4275305 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
GZS16_RS20465 4275445..4276401 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
GZS16_RS20470 4276711..4277241 + 531 WP_000055072.1 inorganic diphosphatase -
GZS16_RS20475 4277321..4277671 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
GZS16_RS20480 4277665..4277916 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
GZS16_RS20485 4278128..4278469 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
GZS16_RS20490 4278472..4282251 - 3780 WP_000060978.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T149011 WP_000239579.1 NZ_CP048310:c4277671-4277321 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T149011 NZ_CP063915:c5221789-5221514 [Klebsiella variicola]
ATGGAGCAGCAGGTTTTATCTTTACGTAATAAACAGCGCCACACGCTGGAGCAACTGTTTAAAATCCCTGTGCCACAGGG
CATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGCGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCATCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT149011 WP_001223208.1 NZ_CP048310:c4277916-4277665 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT149011 NZ_CP063915:c5221517-5221158 [Klebsiella variicola]
ATGATCAAACCTAAAACGCCAAACTCAATGGAGATTGCCGGCCAGCCTGCCGTTATCAATTACGTGCCTGAGCTCAATGC
ATTCCGCGGTAAGTTCCTTGGATTATCAGGATATTGCGATTTTGTTTCCGATAGTATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAATATCTCGCAGATTGTCATGAAGCGGGGATTGAGCCTTATGCTCACCCGGAAAAGATGAAAACC
TTCACGCTGCGCTATCCTGAGTCATTTGGCGAGCGTCTTAGCTCCGCCGCTGCGGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References