Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1893421..1894256 Replicon chromosome
Accession NZ_CP048310
Organism Escherichia coli strain 32-4

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7I6H0K3
Locus tag GZS16_RS08895 Protein ID WP_000854760.1
Coordinates 1893421..1893798 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag GZS16_RS08900 Protein ID WP_001295723.1
Coordinates 1893888..1894256 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS16_RS08855 1888941..1889414 + 474 WP_001105407.1 DNA gyrase inhibitor SbmC -
GZS16_RS08860 1889612..1890670 + 1059 WP_001200895.1 FUSC family protein -
GZS16_RS08865 1890842..1891171 + 330 WP_000450409.1 DUF496 family protein -
GZS16_RS08870 1891272..1891454 - 183 WP_024174802.1 ethanolamine utilization protein -
GZS16_RS25145 1891908..1892297 - 390 WP_001445118.1 IS110 family transposase -
GZS16_RS08885 1893095..1893208 - 114 WP_032140621.1 DUF957 domain-containing protein -
GZS16_RS08890 1893221..1893424 - 204 WP_000761705.1 hypothetical protein -
GZS16_RS08895 1893421..1893798 - 378 WP_000854760.1 TA system toxin CbtA family protein Toxin
GZS16_RS08900 1893888..1894256 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GZS16_RS08905 1894419..1894640 - 222 WP_000692345.1 DUF987 domain-containing protein -
GZS16_RS08910 1894709..1895188 - 480 Protein_1743 RadC family protein -
GZS16_RS08915 1895204..1895676 - 473 Protein_1744 antirestriction protein -
GZS16_RS08920 1895771..1896016 - 246 WP_162676367.1 antirestriction protein -
GZS16_RS25150 1896016..1896832 - 817 Protein_1746 DUF945 domain-containing protein -
GZS16_RS08935 1896951..1897646 - 696 Protein_1747 IS1 family transposase -
GZS16_RS08940 1897702..1898332 + 631 Protein_1748 IS30 family transposase -
GZS16_RS08945 1898344..1898589 - 246 Protein_1749 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1887625..1888845 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14165.19 Da        Isoelectric Point: 7.3249

>T148996 WP_000854760.1 NZ_CP048310:c1893798-1893421 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T148996 NZ_CP063913:2874100-2874585 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGCCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT148996 WP_001295723.1 NZ_CP048310:c1894256-1893888 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT148996 NZ_CP063913:2873810-2874103 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H0K3

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References