Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 765149..765983 Replicon chromosome
Accession NZ_CP048310
Organism Escherichia coli strain 32-4

Toxin (Protein)


Gene name yeeV Uniprot ID W0S379
Locus tag GZS16_RS03650 Protein ID WP_000854808.1
Coordinates 765606..765983 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2J4HRK6
Locus tag GZS16_RS03645 Protein ID WP_001354276.1
Coordinates 765149..765517 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS16_RS03625 762810..763628 + 819 WP_001234702.1 DUF945 domain-containing protein -
GZS16_RS03630 763719..764204 + 486 WP_000214319.1 antirestriction protein -
GZS16_RS03635 764220..764696 + 477 WP_001354275.1 RadC family protein -
GZS16_RS03640 764765..764986 + 222 WP_000692345.1 DUF987 domain-containing protein -
GZS16_RS03645 765149..765517 + 369 WP_001354276.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
GZS16_RS03650 765606..765983 + 378 WP_000854808.1 type IV toxin-antitoxin system toxin CbtA Toxin
GZS16_RS03655 765980..766468 + 489 WP_000761645.1 hypothetical protein -
GZS16_RS03660 766480..766677 + 198 WP_000839241.1 DUF957 domain-containing protein -
GZS16_RS03665 766762..767607 + 846 WP_001280440.1 DUF4942 domain-containing protein -
GZS16_RS03675 767906..768412 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
GZS16_RS03680 768491..770332 - 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13909.93 Da        Isoelectric Point: 7.9085

>T148990 WP_000854808.1 NZ_CP048310:765606-765983 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T148990 NZ_CP063913:880487-880705 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13649.47 Da        Isoelectric Point: 7.0261

>AT148990 WP_001354276.1 NZ_CP048310:765149-765517 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Download         Length: 369 bp

>AT148990 NZ_CP063913:880086-880460 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W0S379

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4HRK6

References