Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 84317..84814 Replicon plasmid p009_C
Accession NZ_CP048307
Organism Escherichia coli strain 9

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GZS17_RS25610 Protein ID WP_001173343.1
Coordinates 84641..84814 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag GZS17_RS25605 Protein ID WP_001312331.1
Coordinates 84317..84613 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS17_RS25585 81046..81765 - 720 WP_000611291.1 two-component system response regulator DcuR -
GZS17_RS25590 81762..83393 - 1632 WP_021566752.1 sensor histidine kinase -
GZS17_RS25595 83574..83804 + 231 WP_000371715.1 (4Fe-4S)-binding protein -
GZS17_RS25600 83816..84088 + 273 WP_021566753.1 N-acetyltransferase -
GZS17_RS25605 84317..84613 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GZS17_RS25610 84641..84814 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GZS17_RS25615 84933..86450 - 1518 WP_000003806.1 lysine--tRNA ligase -
GZS17_RS25620 86687..88144 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94239 94239


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T148987 WP_001173343.1 NZ_CP048307:84641-84814 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T148987 NZ_CP063912:4969368-4969470 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT148987 WP_001312331.1 NZ_CP048307:84317-84613 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT148987 NZ_CP063912:c4969476-4969332 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References