Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4575050..4575600 Replicon chromosome
Accession NZ_CP048304
Organism Escherichia coli strain 9

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag GZS17_RS21725 Protein ID WP_001160966.1
Coordinates 4575050..4575364 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag GZS17_RS21730 Protein ID WP_000125566.1
Coordinates 4575367..4575600 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS17_RS21700 4570725..4572011 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
GZS17_RS21705 4572011..4573000 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GZS17_RS21710 4572997..4573818 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GZS17_RS21715 4573821..4574198 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GZS17_RS21720 4574205..4575053 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GZS17_RS21725 4575050..4575364 - 315 WP_001160966.1 CcdB family protein Toxin
GZS17_RS21730 4575367..4575600 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GZS17_RS21735 4575686..4576165 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
GZS17_RS21740 4576357..4578219 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
GZS17_RS21745 4578212..4578742 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GZS17_RS21750 4578850..4580181 - 1332 WP_001183207.1 MFS transporter -
GZS17_RS21755 4580240..4580527 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T148980 WP_001160966.1 NZ_CP048304:c4575364-4575050 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T148980 NZ_CP048304:c4575364-4575050 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT148980 WP_000125566.1 NZ_CP048304:c4575600-4575367 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT148980 NZ_CP048304:c4575600-4575367 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References