Detailed information of TA system TA148979

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4375927..4376725
• Replicon: chromosome
• Accession: NZ_CP048304
• Organism: Escherichia coli strain 9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: GZS17_RS20805
• Protein ID: WP_000854737.1
• Coordinates: 4376348..4376725 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: GZS17_RS20800
• Protein ID: WP_001285481.1
• Coordinates: 4375927..4376301 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS17_RS20760	4371902..4372354	+	453	WP_000682723.1	hypothetical protein	-
GZS17_RS20765	4372472..4372705	+	234	WP_001213776.1	DUF905 family protein	-
GZS17_RS20770	4372805..4373626	+	822	WP_162655256.1	DUF945 domain-containing protein	-
GZS17_RS20775	4373626..4373871	+	246	WP_001164966.1	hypothetical protein	-
GZS17_RS20780	4373965..4374438	+	474	WP_001313575.1	antirestriction protein	-
GZS17_RS20785	4374454..4374930	+	477	WP_001186170.1	RadC family protein	-
GZS17_RS20790	4374993..4375214	+	222	WP_000692312.1	DUF987 domain-containing protein	-
GZS17_RS20795	4375233..4375877	+	645	WP_001617449.1	hypothetical protein	-
GZS17_RS20800	4375927..4376301	+	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS17_RS20805	4376348..4376725	+	378	WP_000854737.1	TA system toxin CbtA family protein	Toxin
GZS17_RS20810	4376722..4377214	+	493	Protein_4063	hypothetical protein	-
GZS17_RS20815	4377293..4378281	-	989	Protein_4064	IS630 family transposase	-
GZS17_RS20820	4378429..4378617	-	189	Protein_4065	IS66 family transposase	-
GZS17_RS20825	4378678..4379811	+	1134	WP_000555401.1	IS110-like element ISEc45 family transposase	-
GZS17_RS20830	4380065..4381469	-	1405	Protein_4067	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14240.26 Da
• Isoelectric Point: 7.8051

>T148979 WP_000854737.1 NZ_CP048304:4376348-4376725 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T148979 NZ_CP063912:c1594872-1594555 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT148979 WP_001285481.1 NZ_CP048304:4375927-4376301 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT148979 NZ_CP063912:c1594562-1594275 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0P0SQV8