Detailed information of TA system TA148960

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 458647..459438
• Replicon: chromosome
• Accession: NZ_CP048304
• Organism: Escherichia coli strain 9

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: GZS17_RS02140
• Protein ID: WP_000691791.1
• Coordinates: 458647..459072 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: GZS17_RS02145
• Protein ID: WP_000939437.1
• Coordinates: 459103..459438 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZS17_RS02115	454313..454810	-	498	WP_000509808.1	hypothetical protein	-
GZS17_RS02120	455492..455878	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
GZS17_RS02125	456501..457232	-	732	WP_001216639.1	TIGR02646 family protein	-
GZS17_RS02130	457229..457450	-	222	WP_125112077.1	hypothetical protein	-
GZS17_RS02135	457435..458626	-	1192	Protein_398	AAA family ATPase	-
GZS17_RS02140	458647..459072	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
GZS17_RS02145	459103..459438	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GZS17_RS02150	459438..459911	-	474	WP_001292742.1	DNA repair protein RadC	-
GZS17_RS02155	459941..460755	-	815	Protein_402	DUF945 domain-containing protein	-
GZS17_RS02160	460991..461944	-	954	WP_000290406.1	hypothetical protein	-
GZS17_RS02165	462537..463151	+	615	WP_000772910.1	inovirus Gp2 family protein	-
GZS17_RS02170	463269..463487	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	458647..468251	9604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T148960 WP_000691791.1 NZ_CP048304:c459072-458647 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T148960 NZ_CP063910:c1537525-1537193 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGTGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT148960 WP_000939437.1 NZ_CP048304:c459438-459103 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT148960 NZ_CP063910:c1537783-1537526 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6