Detailed information of TA system TA148956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 138218..138750
• Replicon: plasmid pRle248b
• Accession: NZ_CP048284
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: GYN07_RS32840
• Protein ID: WP_020047919.1
• Coordinates: 138218..138511 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GYN07_RS32845
• Protein ID: WP_026264729.1
• Coordinates: 138508..138750 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS32825	134073..136043	-	1971	WP_020047922.1	Fe(3+)-hydroxamate ABC transporter permease FhuB	-
GYN07_RS32830	136043..136921	-	879	WP_020047921.1	iron-siderophore ABC transporter substrate-binding protein	-
GYN07_RS32835	136935..137729	-	795	WP_032999057.1	ATP-binding cassette domain-containing protein	-
GYN07_RS32840	138218..138511	-	294	WP_020047919.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYN07_RS32845	138508..138750	-	243	WP_026264729.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
GYN07_RS32850	138916..139818	-	903	WP_020047917.1	MBL fold metallo-hydrolase	-
GYN07_RS34875	139796..139933	+	138	WP_155247457.1	hypothetical protein	-
GYN07_RS32855	139921..140259	-	339	Protein_131	DsbA family protein	-
GYN07_RS32860	140256..140984	+	729	Protein_132	substrate binding domain-containing protein	-
GYN07_RS32865	141157..141420	+	264	Protein_133	acylphosphatase	-
GYN07_RS32870	141580..142232	+	653	Protein_134	Rieske 2Fe-2S domain-containing protein	-
GYN07_RS32875	142229..143075	+	847	Protein_135	aromatic ring-hydroxylating dioxygenase subunit alpha	-
GYN07_RS32880	143072..143731	+	660	WP_020047912.1	glutathione S-transferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..288152	288152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (3-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11108.60 Da
• Isoelectric Point: 5.5404

>T148956 WP_020047919.1 NZ_CP048284:c138511-138218 [Rhizobium leguminosarum bv. viciae 248]
MSFKLSAEAEGDIIAIAEQGVRPFGAGQAKRYHDELFALFDLIAANPRIARERDEIDPPVRIHPFKAHLIVYRIENDETI
FVIRIRHAHEDWASDSI

Nucleotide

• Length: 294 bp

>T148956 NZ_CP063909:c76018-75548 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8811.96 Da
• Isoelectric Point: 9.7320

>AT148956 WP_026264729.1 NZ_CP048284:c138750-138508 [Rhizobium leguminosarum bv. viciae 248]
MATMNVSLPGPMKDWVEAQAKTGRYSNASDYVRDLIRRDQARNDKIAAMQSFVEAGLQSGVGVRSKDELFAEAMARTSKS

Nucleotide

• Length: 243 bp

>AT148956 NZ_CP063909:c76458-76015 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA