Detailed information of TA system TA148953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 204224..205026
• Replicon: plasmid pRle248e
• Accession: NZ_CP048281
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: GYN07_RS26110
• Protein ID: WP_020046931.1
• Coordinates: 204224..204751 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: A0A1B1CH27
• Locus tag: GYN07_RS26115
• Protein ID: WP_020046930.1
• Coordinates: 204748..205026 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS26095	199449..200573	-	1125	WP_020046934.1	DNA repair exonuclease	-
GYN07_RS26100	200881..202272	-	1392	WP_026264454.1	aldehyde dehydrogenase family protein	-
GYN07_RS26105	202274..203920	-	1647	WP_020046932.1	acetolactate synthase large subunit	-
GYN07_RS26110	204224..204751	-	528	WP_020046931.1	hypothetical protein	Toxin
GYN07_RS26115	204748..205026	-	279	WP_020046930.1	DUF1778 domain-containing protein	Antitoxin
GYN07_RS26120	205248..206126	-	879	WP_026264453.1	hypothetical protein	-
GYN07_RS26125	206554..207276	-	723	WP_020046929.1	ribonuclease activity regulator RraA	-
GYN07_RS26130	207347..208132	-	786	WP_020046928.1	IclR family transcriptional regulator	-
GYN07_RS26135	208347..209867	+	1521	WP_020046927.1	peptide ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..727585	727585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18732.76 Da
• Isoelectric Point: 10.1883

>T148953 WP_020046931.1 NZ_CP048281:c204751-204224 [Rhizobium leguminosarum bv. viciae 248]
VTLPVWHGEPIAKSHDRAAFDCGDAAMNEFIRRFARQSHEQNAAKTFCAIDKAQPDRILGFYTVAPSAVTHEAVPPKLTR
GLARHEVAGFKLARLATDIKVAGKGLGGQFIAAAALRCLRLASDGGGILLIIDAKSKRAAHWYASYGAEPLQGKPLTLIM
PLATFAADFKAKGLL

Nucleotide

• Length: 528 bp

>T148953 NZ_CP063908:4700385-4700669 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10189.87 Da
• Isoelectric Point: 8.6184

>AT148953 WP_020046930.1 NZ_CP048281:c205026-204748 [Rhizobium leguminosarum bv. viciae 248]
MPRTADNKTERYQLRIPPKQKAMIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLEHPPKPNAK
LRAAISALPDAL

Nucleotide

• Length: 279 bp

>AT148953 NZ_CP063908:4700153-4700395 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1B1CH27