Detailed information of TA system TA148951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5066763..5067394
• Replicon: chromosome
• Accession: NZ_CP048280
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GYN07_RS25035
• Protein ID: WP_020052604.1
• Coordinates: 5066763..5067155 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R0AC40
• Locus tag: GYN07_RS25040
• Protein ID: WP_020052605.1
• Coordinates: 5067152..5067394 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS25010	5062333..5063097	-	765	WP_020052599.1	SDR family oxidoreductase	-
GYN07_RS25015	5063226..5064131	+	906	WP_020052600.1	LysR family transcriptional regulator	-
GYN07_RS25020	5064133..5065011	-	879	WP_026265557.1	LysR family transcriptional regulator	-
GYN07_RS25025	5065102..5066352	+	1251	WP_020052602.1	MFS transporter	-
GYN07_RS25030	5066349..5066735	+	387	WP_020052603.1	VOC family protein	-
GYN07_RS25035	5066763..5067155	-	393	WP_020052604.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYN07_RS25040	5067152..5067394	-	243	WP_020052605.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GYN07_RS25045	5067448..5069109	-	1662	WP_020052606.1	urocanate hydratase	-
GYN07_RS25050	5069315..5069644	-	330	WP_020052607.1	TfoX/Sxy family protein	-
GYN07_RS25055	5069648..5070745	-	1098	WP_020052608.1	DUF917 family protein	-
GYN07_RS25060	5070726..5072006	-	1281	WP_020052609.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-121)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14587.61 Da
• Isoelectric Point: 5.7723

>T148951 WP_020052604.1 NZ_CP048280:c5067155-5066763 [Rhizobium leguminosarum bv. viciae 248]
MKYLLDANAVIALMKGNERFVAELRKHRPQDFGVPTIVAHELFYGAYKSLRTDENLARIDALQFNILEFDRDDARKAGEI
RAALQASGTPIGPYDVLIAGQAAARGLILITRNLREFERAADLQTENWEG

Nucleotide

• Length: 393 bp

>T148951 NZ_CP063908:c3825050-3824682 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9051.12 Da
• Isoelectric Point: 4.3322

>AT148951 WP_020052605.1 NZ_CP048280:c5067394-5067152 [Rhizobium leguminosarum bv. viciae 248]
METAKIFWSGRSQAVRLPKEFRFDGDSVAIRRQGRAIILEPISGDWEWLADVTGPVDPDFATAAEEQPAGQERPELDFFK

Nucleotide

• Length: 243 bp

>AT148951 NZ_CP063908:c3825268-3825047 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4R0AC40