Detailed information of TA system TA148950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4782936..4783525
• Replicon: chromosome
• Accession: NZ_CP048280
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: GYN07_RS23560
• Protein ID: WP_020052327.1
• Coordinates: 4782936..4783133 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1L3ZE25
• Locus tag: GYN07_RS23565
• Protein ID: WP_020052328.1
• Coordinates: 4783130..4783525 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS23535	4778651..4779286	+	636	WP_020052324.1	trimeric intracellular cation channel family protein	-
GYN07_RS23540	4779505..4779924	-	420	WP_003555773.1	transcriptional repressor	-
GYN07_RS23545	4780226..4780741	+	516	WP_011649939.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
GYN07_RS23550	4780787..4782019	+	1233	WP_017962727.1	beta-ketoacyl-ACP synthase I	-
GYN07_RS23555	4782027..4782833	+	807	WP_020052326.1	enoyl-ACP reductase FabI	-
GYN07_RS23560	4782936..4783133	+	198	WP_020052327.1	type II toxin-antitoxin system HicA family toxin	Toxin
GYN07_RS23565	4783130..4783525	+	396	WP_020052328.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GYN07_RS23570	4783529..4784545	-	1017	WP_020052329.1	class I SAM-dependent methyltransferase	-
GYN07_RS23575	4784621..4786759	-	2139	WP_026265531.1	polyribonucleotide nucleotidyltransferase	-
GYN07_RS23580	4787108..4787377	-	270	WP_003544317.1	30S ribosomal protein S15	-
GYN07_RS23585	4787531..4788382	-	852	WP_026265532.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7195.35 Da
• Isoelectric Point: 11.4650

>T148950 WP_020052327.1 NZ_CP048280:4782936-4783133 [Rhizobium leguminosarum bv. viciae 248]
VERNSQKIIARLKREGFEVVSIKGSHHKLRRGNQTIIVPHPKKDLPIGTALAIAKQAGWNASDKS

Nucleotide

• Length: 198 bp

>T148950 NZ_CP063908:c3420798-3420313 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14472.30 Da
• Isoelectric Point: 4.4015

>AT148950 WP_020052328.1 NZ_CP048280:4783130-4783525 [Rhizobium leguminosarum bv. viciae 248]
MKYFIALVHKDRDSAFGISFPDLPSVFSAADEEEDLTANAIEALRLWAEDETLPMPSSYDEISSKQDIREQLAAGAFLTR
IPFIEDATRTVRANVTFDKGMLDAIDKAAKERGLTRSAFLASAARKEIEAA

Nucleotide

• Length: 396 bp

>AT148950 NZ_CP063908:c3421088-3420795 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1L3ZE25