Detailed information of TA system TA148947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4238641..4239203
• Replicon: chromosome
• Accession: NZ_CP048280
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GYN07_RS21060
• Protein ID: WP_020051926.1
• Coordinates: 4238641..4238886 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GYN07_RS21065
• Protein ID: WP_020051927.1
• Coordinates: 4238886..4239203 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS21025	4233696..4234262	-	567	WP_020051919.1	hypothetical protein	-
GYN07_RS21030	4234366..4235817	+	1452	WP_020051920.1	hypothetical protein	-
GYN07_RS21035	4235817..4236173	+	357	WP_020051921.1	hypothetical protein	-
GYN07_RS21040	4236305..4237066	+	762	WP_020051922.1	hypothetical protein	-
GYN07_RS21045	4237066..4237311	+	246	WP_020051923.1	hypothetical protein	-
GYN07_RS21050	4237482..4237916	+	435	WP_020051924.1	hypothetical protein	-
GYN07_RS21055	4237924..4238334	+	411	WP_020051925.1	hypothetical protein	-
GYN07_RS21060	4238641..4238886	+	246	WP_020051926.1	BrnT family toxin	Toxin
GYN07_RS21065	4238886..4239203	+	318	WP_020051927.1	BrnA antitoxin family protein	Antitoxin
GYN07_RS21070	4239826..4241223	+	1398	WP_050565873.1	ethanolamine ammonia-lyase subunit EutB	-
GYN07_RS21075	4241220..4241966	+	747	WP_020051929.1	ethanolamine ammonia-lyase subunit EutC	-
GYN07_RS21080	4241997..4243361	+	1365	WP_020051930.1	ethanolamine permease	-
GYN07_RS21085	4243517..4244035	-	519	WP_020051931.1	DUF2937 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4193146..4239203	46057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-103)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9036.59 Da
• Isoelectric Point: 8.7696

>T148947 WP_020051926.1 NZ_CP048280:4238641-4238886 [Rhizobium leguminosarum bv. viciae 248]
MKLVWDEPKRIANIEKHGLDFADLTFEFFLSSVVVPAKDGRSKAIGRLADGTMVVIFVNLGSEALSIISMRPARKDERSM
I

Nucleotide

• Length: 246 bp

>T148947 NZ_CP063908:1410052-1411374 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCTCTCTCACTGCCGCTGCAGTATGGCAATATCACGG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAA
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTTACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11846.69 Da
• Isoelectric Point: 10.5975

>AT148947 WP_020051927.1 NZ_CP048280:4238886-4239203 [Rhizobium leguminosarum bv. viciae 248]
MIIKARKMTQFQPGHGYSKEDWDAVSDNPPLSKEEMARAKPFKEAFPDVAKKMEKAIAARGRPKLDNPKQSLNIRLDADI
VQFYKATGKGWQSRMNDALRKAAGL

Nucleotide

• Length: 318 bp

>AT148947 NZ_CP063908:1409786-1410052 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA