Detailed information of TA system TA148946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 3333021..3333534
• Replicon: chromosome
• Accession: NZ_CP048280
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: GYN07_RS16730
• Protein ID: WP_080646665.1
• Coordinates: 3333304..3333534 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A4R0BKL9
• Locus tag: GYN07_RS16725
• Protein ID: WP_020051161.1
• Coordinates: 3333021..3333311 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS16710	3328676..3329590	-	915	WP_020051158.1	HD-GYP domain-containing protein	-
GYN07_RS16715	3330072..3331175	+	1104	WP_020051159.1	branched-chain amino acid ABC transporter substrate-binding protein	-
GYN07_RS16720	3331295..3332992	+	1698	WP_026265388.1	adenine deaminase	-
GYN07_RS16725	3333021..3333311	-	291	WP_020051161.1	putative addiction module antidote protein	Antitoxin
GYN07_RS16730	3333304..3333534	-	231	WP_080646665.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYN07_RS16735	3333653..3335299	-	1647	WP_020051163.1	alpha-glucosidase family protein	-
GYN07_RS16740	3335586..3337166	+	1581	WP_020051164.1	peptide ABC transporter substrate-binding protein	-
GYN07_RS16745	3337213..3338136	+	924	WP_020051165.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-71)

Antitoxin

• Uncharacterized protein HI1420 (10-93)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8404.49 Da
• Isoelectric Point: 7.8528

>T148946 WP_080646665.1 NZ_CP048280:c3333534-3333304 [Rhizobium leguminosarum bv. viciae 248]
IALRLQRLATGHAGDASPIGEGLSELRIHYGPGYRIYYCKRGETIIVLLCGGDKSTQETDIRTAKRIASEWRDDNG

Nucleotide

• Length: 231 bp

>T148946 NZ_CP063908:c37808-37590 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10344.90 Da
• Isoelectric Point: 4.8499

>AT148946 WP_020051161.1 NZ_CP048280:c3333311-3333021 [Rhizobium leguminosarum bv. viciae 248]
MAEEIFDFDPAEMLDSDEAIEAFIIEALQTGDAKFIASALGVVARAKGMSKIARKTGLAREHLYKALSENGNPTLETTLA
VMKAIGLELTTKHHAV

Nucleotide

• Length: 291 bp

>AT148946 NZ_CP063908:c38209-37835 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A4R0BKL9