Detailed information of TA system TA148945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2952537..2953210
• Replicon: chromosome
• Accession: NZ_CP048280
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GYN07_RS14755
• Protein ID: WP_020050800.1
• Coordinates: 2952537..2952950 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GYN07_RS14760
• Protein ID: WP_020050801.1
• Coordinates: 2952947..2953210 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS14745	2949951..2951147	-	1197	WP_020050798.1	efflux RND transporter periplasmic adaptor subunit	-
GYN07_RS14750	2951416..2952357	-	942	WP_020050799.1	adenylate/guanylate cyclase domain-containing protein	-
GYN07_RS14755	2952537..2952950	-	414	WP_020050800.1	type II toxin-antitoxin system VapC family toxin	Toxin
GYN07_RS14760	2952947..2953210	-	264	WP_020050801.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GYN07_RS14765	2953299..2954849	-	1551	WP_020050802.1	SpoVR family protein	-
GYN07_RS14770	2954846..2956120	-	1275	WP_020050803.1	YeaH/YhbH family protein	-
GYN07_RS14775	2956133..2958076	-	1944	WP_020050804.1	PrkA family serine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-54)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15311.73 Da
• Isoelectric Point: 5.6789

>T148945 WP_020050800.1 NZ_CP048280:c2952950-2952537 [Rhizobium leguminosarum bv. viciae 248]
MTAWMLDTNMVSHVIKGDRPEIIDRLAALPMEDIVISSITEGELFYGLAKRGYPKVLSERIRQFLLRVDVLPWDHDVTRT
YGDLRAACEAKGVTLAPLDMMIAAHAVATAATLVTRDKAFARVSEPLKVDDWANERS

Nucleotide

• Length: 414 bp

>T148945 NZ_CP063907:67254-67670 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCATTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10153.26 Da
• Isoelectric Point: 5.1859

>AT148945 WP_020050801.1 NZ_CP048280:c2953210-2952947 [Rhizobium leguminosarum bv. viciae 248]
MATRTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKTGDVILSRKPDSWDGLFELYGKDRVPDDFLGPDDRHQPSHDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT148945 NZ_CP063907:67027-67257 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGTGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA