Detailed information of TA system TA148944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 805438..806933
• Replicon: chromosome
• Accession: NZ_CP048280
• Organism: Rhizobium leguminosarum bv. viciae 248

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: GYN07_RS03885
• Protein ID: WP_020048849.1
• Coordinates: 805438..806706 (-)
• Length: 423 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: GYN07_RS03890
• Protein ID: WP_080646643.1
• Coordinates: 806703..806933 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN07_RS03875	801100..803649	-	2550	WP_196232216.1	DEAD/DEAH box helicase family protein	-
GYN07_RS03880	803763..804542	-	780	WP_020048848.1	SDR family NAD(P)-dependent oxidoreductase	-
GYN07_RS03885	805438..806706	-	1269	WP_020048849.1	type II toxin-antitoxin system HipA family toxin	Toxin
GYN07_RS03890	806703..806933	-	231	WP_080646643.1	helix-turn-helix transcriptional regulator	Antitoxin
GYN07_RS03895	807779..808387	-	609	WP_020048851.1	TetR/AcrR family transcriptional regulator	-
GYN07_RS03900	808499..809185	+	687	WP_020048852.1	PspA/IM30 family protein	-
GYN07_RS03905	809235..809516	+	282	WP_003588051.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 423 a.a.
• Molecular weight: 45572.34 Da
• Isoelectric Point: 5.5482

>T148944 WP_020048849.1 NZ_CP048280:c806706-805438 [Rhizobium leguminosarum bv. viciae 248]
MTSRLLNVWWDGRIVGQFTQDRDGDIGFVYARAWLDDDKTLPLSASLPKRPERFSRRECRPFFGGLLPEESQRLAAARAL
GVSPANDFALLDHLGGDVAGALQLLAEGEAPAGTGSIADRQPVPLDEAGIVAVLEALPVRPLLAGEEGLRLSLAGAQSKL
PVVLVDGRIGLPLPGQATTHILKLPIARFKATTENEAFVMRLAAAIGLDVAAVEPRYVQGRPFLLVERYDRVRGAGGLVH
RIHQEDFCQALSVPPETKYASEGGPTFKDCFDLIRRISARPAGDVLKLLDAAIFNLIAGNADAHGKNFSILYDSQGPRLA
KLYDLLSTVIYPDLSPKLAMRLGQRATLAEMDAEGWKVFAAEAGVGTPLLRRRVNELCDKVLTLTGKVADELAEVSPDRD
PIDQIAGLITDRAEAARLTLQG

Nucleotide

• Length: 1269 bp

>T148944 NZ_CP063907:c66210-65905 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8173.53 Da
• Isoelectric Point: 10.1939

>AT148944 WP_080646643.1 NZ_CP048280:c806933-806703 [Rhizobium leguminosarum bv. viciae 248]
MPKHVQSPADIGALVRSTRKEQKLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSLDILAPGERRQ

Nucleotide

• Length: 231 bp

>AT148944 NZ_CP063907:c66430-66212 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA