Detailed information of TA system TA148940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1960035..1960559
• Replicon: chromosome
• Accession: NZ_CP048279
• Organism: Staphylococcus sp. MI 10-1553

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GZH82_RS09100
• Protein ID: WP_162682229.1
• Coordinates: 1960035..1960388 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A380G7B9
• Locus tag: GZH82_RS09105
• Protein ID: WP_019169265.1
• Coordinates: 1960389..1960559 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZH82_RS09080	1957343..1958113	-	771	WP_162682225.1	RNA polymerase sigma factor SigB	-
GZH82_RS09085	1958088..1958567	-	480	WP_203232800.1	anti-sigma B factor RsbW	-
GZH82_RS09090	1958567..1958893	-	327	WP_162682227.1	anti-sigma factor antagonist	-
GZH82_RS09095	1958973..1959977	-	1005	WP_162682228.1	PP2C family protein-serine/threonine phosphatase	-
GZH82_RS09100	1960035..1960388	-	354	WP_162682229.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GZH82_RS09105	1960389..1960559	-	171	WP_019169265.1	hypothetical protein	Antitoxin
GZH82_RS09110	1960644..1961792	-	1149	WP_162682230.1	alanine racemase	-
GZH82_RS09115	1961826..1962176	-	351	WP_162682231.1	holo-ACP synthase	-
GZH82_RS09120	1962173..1962691	-	519	WP_162682232.1	PH domain-containing protein	-
GZH82_RS09125	1962675..1964204	-	1530	WP_162682233.1	PH domain-containing protein	-
GZH82_RS09130	1964197..1964670	-	474	WP_162682234.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13098.30 Da
• Isoelectric Point: 10.4439

>T148940 WP_162682229.1 NZ_CP048279:c1960388-1960035 [Staphylococcus sp. MI 10-1553]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSDKKMKEVNNAIGISLGLHINHHK

Nucleotide

• Length: 354 bp

>T148940 NZ_CP063906:3316758-3317042 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCTATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6371.97 Da
• Isoelectric Point: 3.9930

>AT148940 WP_019169265.1 NZ_CP048279:c1960559-1960389 [Staphylococcus sp. MI 10-1553]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYSVECEACDCNESHLTSDYKED

Nucleotide

• Length: 171 bp

>AT148940 NZ_CP063906:3316526-3316768 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A380G7B9