Detailed information of TA system TA148938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3762727..3763544
• Replicon: chromosome
• Accession: NZ_CP048275
• Organism: Morganella morganii strain N18-00103

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A2C5TM43
• Locus tag: GYM50_RS18095
• Protein ID: WP_004236532.1
• Coordinates: 3762727..3763209 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: J7TDK6
• Locus tag: GYM50_RS18100
• Protein ID: WP_004236533.1
• Coordinates: 3763236..3763544 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM50_RS18065	3758086..3758709	-	624	WP_004236525.1	thiol:disulfide interchange protein DsbA	-
GYM50_RS18070	3758728..3759723	-	996	WP_004236526.1	serine/threonine protein kinase	-
GYM50_RS18075	3759723..3759992	-	270	WP_004240741.1	YihD family protein	-
GYM50_RS18080	3760195..3761310	+	1116	WP_087825438.1	molybdenum cofactor guanylyltransferase MobA	-
GYM50_RS18085	3761525..3761827	+	303	WP_004240743.1	exported protein precursor	-
GYM50_RS18090	3761955..3762476	+	522	WP_015422407.1	isopentenyl-diphosphate Delta-isomerase	-
GYM50_RS19040	3762473..3762613	+	141	WP_004236531.1	hypothetical protein	-
GYM50_RS18095	3762727..3763209	-	483	WP_004236532.1	GNAT family N-acetyltransferase	Toxin
GYM50_RS18100	3763236..3763544	-	309	WP_004236533.1	DUF1778 domain-containing protein	Antitoxin
GYM50_RS18105	3763676..3765028	-	1353	WP_046024838.1	glutathione-disulfide reductase	-
GYM50_RS18110	3765098..3765940	-	843	WP_036417394.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
GYM50_RS18115	3766130..3766519	+	390	WP_004236536.1	DUF1090 domain-containing protein	-
GYM50_RS18120	3766931..3767923	+	993	WP_087825439.1	inner membrane protein YhjD	-
GYM50_RS18125	3768031..3768237	+	207	WP_004236540.1	4-oxalocrotonate tautomerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (36-135)

Antitoxin

• Vibrio phage ICP1, Orf50 (21-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17729.48 Da
• Isoelectric Point: 10.2370

>T148938 WP_004236532.1 NZ_CP048275:c3763209-3762727 [Morganella morganii]
MQQHHDFTVFQSGEESLNHWLKTRALQNQQSGASRTFVVCHHNRIMAYYALSTGVITCSQAAGRFRRNMPPEIPVILPGR
LAVDESVKGRGIGRGLIKDAALRVLQAAGIVGIRGIVVRALSDNARRFYEHTGFMPSPADPMLLMITLRDLQLATGIYSE

Nucleotide

• Length: 483 bp

>T148938 NZ_CP063906:2837382-2837765 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11499.15 Da
• Isoelectric Point: 8.6397

>AT148938 WP_004236533.1 NZ_CP048275:c3763544-3763236 [Morganella morganii]
MTKHTDEQHPVSGVNITRSTLNLRIKPEDKILIDRAAHAAGKNRTEFVLEAARRAAEETLADLRVIDVSTDVYQQFITRL
DMAPQSGEALKKTMLTKAPWEK

Nucleotide

• Length: 309 bp

>AT148938 NZ_CP063906:2837140-2837382 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A2C5TM43

Antitoxin

• AlphaFold DB: J7TDK6