148936

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 5230133..5230786 Replicon chromosome
Accession NZ_CP048274
Organism Brevibacillus sp. NSP2.1

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag M655_RS25720 Protein ID WP_005830205.1
Coordinates 5230133..5230483 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3M8AYL7
Locus tag M655_RS25725 Protein ID WP_025845698.1
Coordinates 5230487..5230786 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M655_RS25695 (M655_025335) 5226868..5227665 - 798 WP_005830213.1 RNA polymerase sigma factor SigB -
M655_RS25700 (M655_025340) 5227634..5228128 - 495 WP_005830211.1 anti-sigma B factor RsbW -
M655_RS25705 (M655_025345) 5228128..5228454 - 327 WP_005830210.1 anti-sigma factor antagonist -
M655_RS25710 (M655_025350) 5228480..5229481 - 1002 WP_007776949.1 PP2C family protein-serine/threonine phosphatase -
M655_RS25715 (M655_025355) 5229475..5229831 - 357 WP_007776946.1 anti-sigma regulatory factor -
M655_RS25720 (M655_025360) 5230133..5230483 - 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M655_RS25725 (M655_025365) 5230487..5230786 - 300 WP_025845698.1 CopG family ribbon-helix-helix protein Antitoxin
M655_RS25730 (M655_025370) 5230965..5232167 - 1203 WP_007776941.1 alanine racemase -
M655_RS25735 (M655_025375) 5232170..5232610 - 441 WP_005830198.1 hypothetical protein -
M655_RS25740 5232949..5233545 - 597 WP_286085930.1 hypothetical protein -
M655_RS25745 5233539..5234063 - 525 WP_286085931.1 hypothetical protein -
M655_RS25750 (M655_025385) 5234270..5235307 - 1038 WP_005830195.1 DUF4367 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T148936 WP_005830205.1 NZ_CP048274:c5230483-5230133 [Brevibacillus sp. NSP2.1]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T148936 NZ_CP063906:c2411783-2411436 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCGGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11323.26 Da        Isoelectric Point: 10.3594

>AT148936 WP_025845698.1 NZ_CP048274:c5230786-5230487 [Brevibacillus sp. NSP2.1]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLVRLVSGV

Download         Length: 300 bp

>AT148936 NZ_CP063906:c2411436-2411137 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGAGACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGCCCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M8AYL7

References