Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4021296..4021932 Replicon chromosome
Accession NZ_CP048273
Organism Bacillus sp. NSP9.1

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag M654_RS20885 Protein ID WP_003179128.1
Coordinates 4021296..4021646 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6I7F8B4
Locus tag M654_RS20890 Protein ID WP_026585993.1
Coordinates 4021651..4021932 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M654_RS20845 4016340..4016939 - 600 WP_026586001.1 SpoIIE family protein phosphatase -
M654_RS20850 4016936..4017727 - 792 WP_026586000.1 RNA polymerase sigma factor SigB -
M654_RS20855 4017693..4018175 - 483 WP_026585999.1 anti-sigma B factor RsbW -
M654_RS20860 4018175..4018501 - 327 WP_026585998.1 anti-sigma factor antagonist -
M654_RS20865 4018560..4019567 - 1008 WP_026585997.1 PP2C family protein-serine/threonine phosphatase -
M654_RS20870 4019578..4019979 - 402 WP_026585996.1 anti-sigma regulatory factor -
M654_RS20875 4019982..4020347 - 366 WP_026585995.1 STAS domain-containing protein -
M654_RS20880 4020351..4021178 - 828 WP_026585994.1 STAS domain-containing protein -
M654_RS20885 4021296..4021646 - 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M654_RS20890 4021651..4021932 - 282 WP_026585993.1 hypothetical protein Antitoxin
M654_RS20895 4022037..4023206 - 1170 WP_026585992.1 alanine racemase -
M654_RS20900 4023414..4024430 - 1017 WP_142246052.1 outer membrane lipoprotein carrier protein LolA -
M654_RS20905 4024707..4025306 + 600 WP_026585990.1 rhomboid family intramembrane serine protease -
M654_RS20910 4025300..4026787 - 1488 WP_026585989.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T148935 WP_003179128.1 NZ_CP048273:c4021646-4021296 [Bacillus sp. NSP9.1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T148935 NZ_CP063906:c1979302-1978892 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10667.07 Da        Isoelectric Point: 4.6920

>AT148935 WP_026585993.1 NZ_CP048273:c4021932-4021651 [Bacillus sp. NSP9.1]
MSESSATTEIMIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT148935 NZ_CP063906:c1979549-1979283 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB A0A6I7F8B4

References