Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 1645..2108 Replicon plasmid unnamed3
Accession NZ_CP048253
Organism Neisseria gonorrhoeae strain CT532

Toxin (Protein)


Gene name VapD Uniprot ID Q51003
Locus tag GYN05_RS11655 Protein ID WP_003690225.1
Coordinates 1830..2108 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID Q51002
Locus tag GYN05_RS11650 Protein ID WP_003690223.1
Coordinates 1645..1830 (+) Length 62 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GYN05_RS11635 (GYN05_11360) 285..1154 + 870 WP_003690219.1 replication initiation protein -
GYN05_RS11640 (GYN05_11365) 1158..1400 + 243 WP_003690220.1 HTH domain-containing protein -
GYN05_RS11645 (GYN05_11370) 1397..1642 + 246 WP_003690221.1 hypothetical protein -
GYN05_RS11650 (GYN05_11375) 1645..1830 + 186 WP_003690223.1 DUF5397 family protein Antitoxin
GYN05_RS11655 (GYN05_11380) 1830..2108 + 279 WP_003690225.1 endoribonuclease VapD Toxin
GYN05_RS11660 (GYN05_11385) 2086..2340 - 255 WP_003690227.1 Cryptic plasmid protein A -
GYN05_RS11665 (GYN05_11390) 2387..3028 - 642 WP_146711211.1 MbeB family mobilization protein -
GYN05_RS11670 (GYN05_11395) 3112..3741 - 630 WP_003696452.1 relaxase/mobilization nuclease domain-containing protein -
GYN05_RS11675 3726..4106 - 381 WP_014580441.1 MobC family plasmid mobilization relaxosome protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..4207 4207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(2-60)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10473.89 Da        Isoelectric Point: 6.9726

>T148927 WP_003690225.1 NZ_CP048253:1830-2108 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Download         Length: 279 bp

>T148927 NZ_CP063904:4379271-4379639 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 62 a.a.        Molecular weight: 6717.54 Da        Isoelectric Point: 4.2641

>AT148927 WP_003690223.1 NZ_CP048253:1645-1830 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Download         Length: 186 bp

>AT148927 NZ_CP063904:4379053-4379274 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5FJI5

Antitoxin

Source ID Structure
AlphaFold DB Q51002

References