Detailed information of TA system TA148925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1577043..1577728
• Replicon: chromosome
• Accession: NZ_CP048250
• Organism: Neisseria gonorrhoeae strain CT532

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q5F6D1
• Locus tag: GYN05_RS08145
• Protein ID: WP_003689143.1
• Coordinates: 1577546..1577728 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q5F6D2
• Locus tag: GYN05_RS08140
• Protein ID: WP_003691454.1
• Coordinates: 1577043..1577444 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYN05_RS08085 (GYN05_07890)	1572072..1572287	-	216	WP_003691538.1	hypothetical protein	-
GYN05_RS08090 (GYN05_07895)	1572339..1572830	-	492	WP_218422917.1	siphovirus Gp157 family protein	-
GYN05_RS08095 (GYN05_07900)	1572827..1573009	-	183	WP_003691535.1	hypothetical protein	-
GYN05_RS08100 (GYN05_07905)	1573149..1573835	-	687	WP_003691532.1	hypothetical protein	-
GYN05_RS08105	1573904..1574065	-	162	WP_003691530.1	hypothetical protein	-
GYN05_RS08110 (GYN05_07910)	1574062..1574337	-	276	WP_050154389.1	hypothetical protein	-
GYN05_RS08115 (GYN05_07915)	1574490..1574822	-	333	WP_003705604.1	hypothetical protein	-
GYN05_RS08120 (GYN05_07920)	1574963..1575256	-	294	WP_250328953.1	hypothetical protein	-
GYN05_RS08125 (GYN05_07925)	1575253..1575729	-	477	WP_002255718.1	hypothetical protein	-
GYN05_RS08130 (GYN05_07930)	1575762..1575962	-	201	WP_047920246.1	hypothetical protein	-
GYN05_RS08135 (GYN05_07935)	1576183..1576944	+	762	WP_012503753.1	hypothetical protein	-
GYN05_RS08140 (GYN05_07940)	1577043..1577444	-	402	WP_003691454.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GYN05_RS08145 (GYN05_07945)	1577546..1577728	-	183	WP_003689143.1	type II toxin-antitoxin system HicA family toxin	Toxin
GYN05_RS08150 (GYN05_07950)	1577898..1578716	-	819	WP_012503752.1	DUF3037 domain-containing protein	-
GYN05_RS08155 (GYN05_07955)	1578713..1579411	-	699	WP_003689139.1	hypothetical protein	-
GYN05_RS08160 (GYN05_07960)	1579650..1580348	-	699	WP_050169364.1	S24 family peptidase	-
GYN05_RS08165 (GYN05_07965)	1580388..1581041	-	654	WP_157149898.1	helix-turn-helix transcriptional regulator	-
GYN05_RS08170	1581290..1581424	+	135	WP_229684436.1	YdaS family helix-turn-helix protein	-
GYN05_RS08175 (GYN05_07975)	1581426..1581626	+	201	WP_012503750.1	hypothetical protein	-
GYN05_RS08180 (GYN05_07980)	1581784..1582011	+	228	WP_003691442.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1561843..1596118	34275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6687.77 Da
• Isoelectric Point: 10.7235

>T148925 WP_003689143.1 NZ_CP048250:c1577728-1577546 [Neisseria gonorrhoeae]
MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKDLPTGTVKNIYKQAGLK

Nucleotide

• Length: 183 bp

>T148925 NZ_CP063904:c3658638-3657721 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14634.43 Da
• Isoelectric Point: 4.5457

>AT148925 WP_003691454.1 NZ_CP048250:c1577444-1577043 [Neisseria gonorrhoeae]
MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAYMHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVM
IEIDPAKISRQQIRFNVSWPQYLLDRVDEYTSANHETRSGFLAKAALLTMNQA

Nucleotide

• Length: 402 bp

>AT148925 NZ_CP063904:c3659402-3658644 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTTGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: Q5F6D1

Antitoxin

• AlphaFold DB: Q5F6D2