Detailed information of TA system TA148921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 97815..99622
• Replicon: plasmid pB61a
• Accession: NZ_CP048244
• Organism: Ewingella americana strain B6-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GXP68_RS21805
• Protein ID: WP_185690472.1
• Coordinates: 98273..99622 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GXP68_RS21800
• Protein ID: WP_185690471.1
• Coordinates: 97815..98276 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXP68_RS21780	94154..95337	-	1184	Protein_90	efflux RND transporter periplasmic adaptor subunit	-
GXP68_RS21785	95741..96100	+	360	WP_034796152.1	helix-turn-helix domain-containing protein	-
GXP68_RS21790	96179..97300	-	1122	WP_185690470.1	NADH:flavin oxidoreductase	-
GXP68_RS21795	97400..97771	+	372	WP_034796154.1	helix-turn-helix transcriptional regulator	-
GXP68_RS21800	97815..98276	-	462	WP_185690471.1	helix-turn-helix domain-containing protein	Antitoxin
GXP68_RS21805	98273..99622	-	1350	WP_185690472.1	HipA domain-containing protein	Toxin
GXP68_RS21810	100170..100679	+	510	WP_185690473.1	hypothetical protein	-
GXP68_RS21815	100730..102394	+	1665	WP_185690474.1	GMC family oxidoreductase	-
GXP68_RS21820	102458..103840	+	1383	WP_185690529.1	cytochrome c	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..331410	331410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-409)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 50910.38 Da
• Isoelectric Point: 4.7882

>T148921 WP_185690472.1 NZ_CP048244:c99622-98273 [Ewingella americana]
MEYLTLQAFLQNQWVDMAHITFPESENNAFRVTELHYDSDYAVENLGRDDNHAVSLNHPVSLFFENDGPRGWLKFLDDII
PSGASRRYWANYLDLEGMAADQQDFVLLRHGTMSPVGNLRIKESLPEYHPQAEQLFFTLDDVKNRASDFLDYAQQRGAAA
GGATGAGGEAPKLLLRCSQHGQIWIDTYQNDSLNRDTHYLVKFPRGSRSEVDCNILRAEFHFYHELAAMGFDTIPTQHMR
LEEGLNYPSLWLPRFDICFADDNQIKRFGMESVYSVLRKAPGTTLYHEEAIRALINKITHSDTVIEQGFDFDIPAFVIEW
VRRDLLNIIFGNSDNHGRNTSLLKDEHSIGLAPIYDFAPMKADPEGIARTTKWSAPLEIGGEYDFAGIAAALDDLVPAET
LLDALRETAQQLLGLKARLVERGVPEQIIKMPGIGFDFIAEKLRRWGLL

Nucleotide

• Length: 1350 bp

>T148921 NZ_CP063904:2776733-2776951 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16954.72 Da
• Isoelectric Point: 10.9518

>AT148921 WP_185690471.1 NZ_CP048244:c98276-97815 [Ewingella americana]
MKSHESAPVSAKETIRKIRERQKLLQNAADSPVEKPATMAESSSRTPSRQRVSKSSTAVSTQDRQAVMHRIIKQLLLGEL
TQGGALKKLRIEVLNLKQDAYAKLVAVSRKTLSDVENDKGNYTSDIINKLFKPFGLQVGLMPVSKQLLSTLLK

Nucleotide

• Length: 462 bp

>AT148921 NZ_CP063904:2776332-2776706 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG