Detailed information of TA system TA148919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 4188426..4189220
• Replicon: chromosome
• Accession: NZ_CP048243
• Organism: Ewingella americana strain B6-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: GXP68_RS19110
• Protein ID: WP_185688734.1
• Coordinates: 4188426..4188947 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A377NHU8
• Locus tag: GXP68_RS19115
• Protein ID: WP_034795311.1
• Coordinates: 4188951..4189220 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXP68_RS19075	4184134..4184721	+	588	WP_034795319.1	ribose 1,5-bisphosphokinase	-
GXP68_RS19080	4184718..4185353	+	636	WP_034795318.1	acetyltransferase	-
GXP68_RS19085	4185377..4186126	+	750	WP_185688730.1	phosphonate metabolism protein PhnP	-
GXP68_RS19090	4186138..4186776	-	639	WP_185688731.1	hypothetical protein	-
GXP68_RS19095	4186970..4187488	+	519	WP_034795315.1	type 1 glutamine amidotransferase	-
GXP68_RS19100	4187577..4188017	-	441	WP_185688732.1	YhbP family protein	-
GXP68_RS19105	4188121..4188417	+	297	WP_185688733.1	GIY-YIG nuclease family protein	-
GXP68_RS19110	4188426..4188947	-	522	WP_185688734.1	GNAT family N-acetyltransferase	Toxin
GXP68_RS19115	4188951..4189220	-	270	WP_034795311.1	DUF1778 domain-containing protein	Antitoxin
GXP68_RS19120	4189397..4189900	-	504	WP_185688735.1	N-acetyltransferase	-
GXP68_RS19125	4189894..4190415	-	522	WP_185688736.1	SCP2 domain-containing protein	-
GXP68_RS19130	4190725..4191720	+	996	WP_185688737.1	U32 family peptidase	-
GXP68_RS19135	4191735..4192613	+	879	WP_185688738.1	U32 family peptidase	-
GXP68_RS19140	4192656..4193825	-	1170	WP_185688739.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-85)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19273.18 Da
• Isoelectric Point: 7.4735

>T148919 WP_185688734.1 NZ_CP048243:c4188947-4188426 [Ewingella americana]
VDDLTIGMLVEDAEYDFSAFDCGEESLNVFLTEHLARQHRNRILRGYLLMTKEPKPKVLGYYTLSGSCFEKASLPSNTQK
RKVPYENVPSITLGRLAVHKELHGQEWGTTLVTHAMKVVYLASKAVGVHALFVDALNEKAKGFYQKLGFIPLAGDNAHSL
FLPTKSIETLFAS

Nucleotide

• Length: 522 bp

>T148919 NZ_CP063904:667836-667938 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10119.56 Da
• Isoelectric Point: 6.4731

>AT148919 WP_034795311.1 NZ_CP048243:c4189220-4188951 [Ewingella americana]
MSALKKQRIDLRLSDADKSAIEEAAAMSNQTITQFMVASASKRAAEVIEHHRRLILNEESWDLVMDAISNPPELNDRLKR
AAKRLENME

Nucleotide

• Length: 270 bp

>AT148919 NZ_CP063904:c667945-667800 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A377NHU8