Detailed information of TA system TA148918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 3972223..3973561
• Replicon: chromosome
• Accession: NZ_CP048243
• Organism: Ewingella americana strain B6-1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: GXP68_RS18140
• Protein ID: WP_185688642.1
• Coordinates: 3972223..3973173 (-)
• Length: 317 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: GXP68_RS18145
• Protein ID: WP_185688643.1
• Coordinates: 3973166..3973561 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXP68_RS18115	3967632..3968396	+	765	WP_185688639.1	chitin disaccharide deacetylase	-
GXP68_RS18120	3968611..3969687	-	1077	WP_185688640.1	PD-(D/E)XK nuclease family protein	-
GXP68_RS18125	3969846..3970841	-	996	WP_185688641.1	hypothetical protein	-
GXP68_RS18130	3970963..3971199	-	237	WP_185690260.1	hypothetical protein	-
GXP68_RS18135	3971641..3971962	+	322	Protein_3551	hypothetical protein	-
GXP68_RS18140	3972223..3973173	-	951	WP_185688642.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GXP68_RS18145	3973166..3973561	-	396	WP_185688643.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
GXP68_RS18150	3973636..3974469	-	834	WP_185688644.1	DUF4942 domain-containing protein	-
GXP68_RS18155	3974583..3974903	-	321	Protein_3555	toxin	-
GXP68_RS18160	3975028..3975606	+	579	WP_185688645.1	inovirus-type Gp2 protein	-
GXP68_RS18165	3975866..3977422	+	1557	WP_185688646.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3968611..3984251	15640

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-303)

Antitoxin

• Protein of unknown function DUF6088 (7-114)

Sequences

Toxin

Protein

• Length: 317 a.a.
• Molecular weight: 36026.27 Da
• Isoelectric Point: 4.6542

>T148918 WP_185688642.1 NZ_CP048243:c3973173-3972223 [Ewingella americana]
MDDLTKLFPDVADALGIESVAIVEKDHYIVELLRLLQPLSFDTHQLVFAGGTALSKAGISLNRMSEDVDIKLVPVAGFSQ
QYSRGQRKSIRKGIIQTIIETIAASGTFRMDEEYPKVTRDEYRYNEIPMRYPQKYSQAPCLRPFIKLELMETDLLEAAEN
RAISSLVMDLTKKGEAVRAFPCATIGSTQAEKLISMMRRTAASMRDVERVVDESLVRHIYDNFCIVREKGADVPALKQFV
QECIEQDIERYGAQYPEFCESPVEELKMGLEELESDPVYKERYLQFVTPMVFSESHVSWEDAYTCFRKTALDVLEA

Nucleotide

• Length: 951 bp

>T148918 NZ_CP063904:514105-514437 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14367.74 Da
• Isoelectric Point: 11.0360

>AT148918 WP_185688643.1 NZ_CP048243:c3973561-3973166 [Ewingella americana]
MTIKARIQSRLKRSKRYVFTRDDFKDLAGYDQIGRALRELVKEGLLLKVGYGIYTKARQNAITGKLMPAAPGGSAAVMIE
ALERLKVDYRLAGATSAYNSGKSTQIPASLEIKTSPRFKRTLSVGSSKLNG

Nucleotide

• Length: 396 bp

>AT148918 NZ_CP063904:513847-514104 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTAGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA