Detailed information of TA system TA148917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3084870..3085786
• Replicon: chromosome
• Accession: NZ_CP048243
• Organism: Ewingella americana strain B6-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GXP68_RS14105
• Protein ID: WP_185688190.1
• Coordinates: 3085328..3085786 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GXP68_RS14100
• Protein ID: WP_185688189.1
• Coordinates: 3084870..3085331 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GXP68_RS14085	3079936..3083115	+	3180	WP_185688188.1	efflux RND transporter permease subunit	-
GXP68_RS14090	3083117..3083473	+	357	WP_202034337.1	copper-binding protein	-
GXP68_RS14095	3083533..3084420	-	888	WP_034788971.1	nucleoside-specific channel-forming protein Tsx	-
GXP68_RS14100	3084870..3085331	+	462	WP_185688189.1	DUF2384 domain-containing protein	Antitoxin
GXP68_RS14105	3085328..3085786	+	459	WP_185688190.1	RES family NAD+ phosphorylase	Toxin
GXP68_RS14110	3085798..3086247	-	450	WP_185688191.1	DMT family transporter	-
GXP68_RS14115	3086250..3086684	-	435	WP_185688192.1	DMT family transporter	-
GXP68_RS14120	3086786..3087727	+	942	WP_185688193.1	LysR family transcriptional regulator	-
GXP68_RS14125	3088455..3088937	-	483	WP_185688194.1	GNAT family N-acetyltransferase	-
GXP68_RS14130	3089042..3090727	-	1686	WP_185688195.1	choline dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-142)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (99-150)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16756.32 Da
• Isoelectric Point: 4.8382

>T148917 WP_185688190.1 NZ_CP048243:3085328-3085786 [Ewingella americana]
VRLYRIVKTKHLSTAWSGTGAQLYGGRWNPPGKPVVYLATSISLAILEIMVHLQSSLLLQSYTLLSIDIPDEKLEQLDLD
SLSEDWKDPLPPDSTQSLGEAWLQSASSVGLRVPSVIVPSEFNVIVNPLHPDFAPLLASVEKQPMAFDPRLI

Nucleotide

• Length: 459 bp

>T148917 NZ_CP063903:67382-67864 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16621.12 Da
• Isoelectric Point: 6.4369

>AT148917 WP_185688189.1 NZ_CP048243:3084870-3085331 [Ewingella americana]
MESKNSELQSKNAPIPAMRLWQSAGLPASGGMSLINAIQKGFSVDSINRLNAELGWSKMALLEIMGINERSFARRKAENG
LLKTEESERVARLIRIVDAATDLFEGDRSAALDWIRRPAPALGGVPPVEMLASEAGALEVNRLIGRLEYGVFG

Nucleotide

• Length: 462 bp

>AT148917 NZ_CP063903:67128-67394 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA