148906

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1004515..1005141 Replicon chromosome
Accession NZ_CP048243
Organism Ewingella americana strain B6-1

Toxin (Protein)


Gene name Hha Uniprot ID A0A2N0MZL7
Locus tag GXP68_RS04685 Protein ID WP_034792706.1
Coordinates 1004515..1004718 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2N0MZ29
Locus tag GXP68_RS04690 Protein ID WP_034792338.1
Coordinates 1004773..1005141 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXP68_RS04665 999692..999940 - 249 WP_185689413.1 hypothetical protein -
GXP68_RS04670 1000129..1001478 - 1350 WP_154145595.1 NCS2 family permease -
GXP68_RS04675 1001547..1003334 - 1788 WP_185689414.1 amidohydrolase family protein -
GXP68_RS04680 1003476..1004423 + 948 WP_185689415.1 LysR family transcriptional regulator -
GXP68_RS04685 1004515..1004718 - 204 WP_034792706.1 hemolysin expression modulator Hha Toxin
GXP68_RS04690 1004773..1005141 - 369 WP_034792338.1 Hha toxicity modulator TomB Antitoxin
GXP68_RS04695 1005382..1005735 - 354 WP_185689416.1 hypothetical protein -
GXP68_RS04700 1006155..1006895 + 741 WP_185690133.1 ABC transporter ATP-binding protein -
GXP68_RS04705 1006892..1007749 + 858 WP_034792334.1 metal ABC transporter permease -
GXP68_RS04710 1007775..1008653 + 879 WP_185689417.1 metal ABC transporter substrate-binding protein -
GXP68_RS04715 1008779..1008919 - 141 WP_072009903.1 type B 50S ribosomal protein L36 -
GXP68_RS04720 1008935..1009195 - 261 WP_185689418.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8084.42 Da        Isoelectric Point: 6.9764

>T148906 WP_034792706.1 NZ_CP048243:c1004718-1004515 [Ewingella americana]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Download         Length: 204 bp

>T148906 NZ_CP063900:3656494-3656775 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGCTTGGCGAGACGGTCAAAAAACAGTTCAAAAA
TAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCAAGC
TTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAAAGA
GAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14278.01 Da        Isoelectric Point: 4.5944

>AT148906 WP_034792338.1 NZ_CP048243:c1005141-1004773 [Ewingella americana]
MDEYSPKRHDIAQLRFLNESLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPDDSPLTDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGEYVCTLMKT

Download         Length: 369 bp

>AT148906 NZ_CP063900:3656262-3656504 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGGCTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGCGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAGCGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N0MZL7

Antitoxin

Source ID Structure
AlphaFold DB A0A2N0MZ29

References