Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 62112..62694 Replicon chromosome
Accession NZ_CP048243
Organism Ewingella americana strain B6-1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag GXP68_RS00310 Protein ID WP_185688981.1
Coordinates 62359..62694 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag GXP68_RS00305 Protein ID WP_154145766.1
Coordinates 62112..62357 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXP68_RS00265 57347..57823 - 477 WP_034794202.1 GNAT family N-acetyltransferase -
GXP68_RS00270 57829..58368 - 540 WP_185690098.1 hypothetical protein -
GXP68_RS00275 58583..59215 + 633 WP_185688976.1 glutathione transferase -
GXP68_RS00280 59276..59722 - 447 WP_185688977.1 GNAT family N-acetyltransferase -
GXP68_RS00285 59737..60369 - 633 WP_185688978.1 LysE family translocator -
GXP68_RS00290 60374..60829 - 456 WP_185688979.1 GNAT family N-acetyltransferase -
GXP68_RS00295 61026..61283 - 258 WP_034794197.1 DUF1471 domain-containing protein -
GXP68_RS00300 61692..61940 + 249 WP_185688980.1 GlsB/YeaQ/YmgE family stress response membrane protein -
GXP68_RS00305 62112..62357 + 246 WP_154145766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GXP68_RS00310 62359..62694 + 336 WP_185688981.1 endoribonuclease MazF Toxin
GXP68_RS00315 62752..63149 - 398 Protein_62 hypothetical protein -
GXP68_RS00320 63221..63919 - 699 WP_185688982.1 urea ABC transporter ATP-binding subunit UrtE -
GXP68_RS00325 63935..64741 - 807 WP_185688983.1 urea ABC transporter ATP-binding protein UrtD -
GXP68_RS00330 64738..65814 - 1077 WP_185688984.1 urea ABC transporter permease subunit UrtC -
GXP68_RS00335 65814..67376 - 1563 WP_202034364.1 urea ABC transporter permease subunit UrtB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-108)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12136.17 Da        Isoelectric Point: 8.5104

>T148902 WP_185688981.1 NZ_CP048243:62359-62694 [Ewingella americana]
MVKPSIPDAGDIIWLDFDPQAGHEQAGHRPAVVLSPAAYNDRVGLLLCCPMTTKIKGYPFEVLIAGIRQSAVLSDQIKSL
DWRKRNAVKKGTVSLLELDEVRAKVKALIDG

Download         Length: 336 bp

>T148902 NZ_CP063900:563666-564988 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGAATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCACTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACTCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCGTGCCG
CAGGCCATCGAAAGCGCCCGCCAAACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
GAAACTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGGGATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8735.08 Da        Isoelectric Point: 4.5183

>AT148902 WP_154145766.1 NZ_CP048243:62112-62357 [Ewingella americana]
MPQVIVKKWGNSPSVRLPVAIMEAASLKVDDTVEIAVEDGKIIIVPVRAKTYSLDSLLGGVTESNMHEEVDFGSPTGREV
L

Download         Length: 246 bp

>AT148902 NZ_CP063900:563400-563666 [Klebsiella quasipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References