Detailed information of TA system TA148901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 405..882
• Replicon: plasmid pASal3
• Accession: NZ_CP048227
• Organism: Aeromonas salmonicida subsp. salmonicida strain J223

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8T8CMR8
• Locus tag: AXA69_RS23110
• Protein ID: WP_073531821.1
• Coordinates: 613..882 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: Q7X2E4
• Locus tag: AXA69_RS23105
• Protein ID: WP_005321784.1
• Coordinates: 405..629 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXA69_RS23105	405..629	+	225	WP_005321784.1	TraY domain-containing protein	Antitoxin
AXA69_RS23110	613..882	+	270	WP_073531821.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXA69_RS23115	991..1620	+	630	WP_005321764.1	hypothetical protein	-
AXA69_RS23120	2198..3223	+	1026	Protein_4	replication initiation protein	-
AXA69_RS23125	3839..4255	+	417	WP_162215864.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7025	7025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Relaxosome protein TraY (4-47)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10358.02 Da
• Isoelectric Point: 10.3800

>T148901 WP_073531821.1 NZ_CP048227:613-882 [Aeromonas salmonicida subsp. salmonicida]
MAWKVELTDTAKKQLARLDKTQSQRITKYLRRIMMLENPRDAGKALTGNLRTYWRYRVGDYRVVCDIRDNDLVIVAVIIG
HRSEVYGGS

Nucleotide

• Length: 270 bp

>T148901 NZ_CP063899:77181-77666 [Klebsiella variicola]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAATATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8686.77 Da
• Isoelectric Point: 4.2712

>AT148901 WP_005321784.1 NZ_CP048227:405-629 [Aeromonas salmonicida subsp. salmonicida]
MLAIRLPSEIEERLANLAQETGRTKTFYAREAILTHLEELEDYYLAERVMENVRKGEERLFSLDDVERDLGLES

Nucleotide

• Length: 225 bp

>AT148901 NZ_CP063899:76927-77193 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A151KEE9